An ultrasensitive ELISA method for the detection of procalcitonin based on magnetic beads and enzyme-antibody labeled gold nanoparticles. Issue 7 (29th January 2016)

• Record Type: Journal Article
• Authors: Liao, Tao; Yuan, Fang; Yu, Haoyang; Li, Zigang
• Is Part Of: Analytical methods. Volume 8:Issue 7(2016)
• Page Start: 1577
• Page End: 1585
• Publication Date: 2016-01-29
• Subjects: Chemistry, Analytic -- Periodicals; Analytical biochemistry -- Periodicals; Chemical laboratories -- Standards -- Periodicals; 543.1905
• DOI: 10.1039/c5ay03298a
• Languages: English
• ISSNs: 1759-9660

Abstract

Abstract : Sepsis is a potentially fatal systemic body infection with a significant mortality rate worldwide. Procalcitonin (PCT) is a specific marker for severe sepsis caused by bacterial infection. Herein, an ultrasensitive ELISA method based on magnetic beads and enzyme-antibody labeled gold nanoparticles was reported for the detection of PCT. Abstract : Sepsis is a potentially fatal systemic body infection with a significant mortality rate worldwide. Procalcitonin (PCT) is a sensitive and specific biomarker for sepsis diagnosis, however, the traditional immune-gold labeling detection of PCT is semi-quantitative with relatively low sensitivity (>0.1 ng mL −1 ). Recently proposed techniques (such as electrochemical immunosensor, microarray, and total internal reflection based methods) require either a complex sensor or sophisticated equipment. Herein, we report a magnetic bead and enzyme-antibody labeled gold nanoparticle based ELISA strategy for sensitive PCT detection. This developed assay is sensitive enough to detect PCT at 20 pg mL −1, efficient for the direct human serum test. Notably, this assay specifically distinguishes PCT from other sepsis markers and the whole assay takes only 1.5 hours to finish.