Copper ion-selective fluorescent sensor using salicyl fluorone immobilized on a glutaraldehyde-coated eggshell membrane. Issue 9 (17th February 2016)

• Record Type: Journal Article
• Authors: Wang, Anyi; Li, Ying; Wang, Taikun; Wang, Shiping
• Is Part Of: Analytical methods. Volume 8:Issue 9(2016)
• Page Start: 2112
• Page End: 2117
• Publication Date: 2016-02-17
• Subjects: Chemistry, Analytic -- Periodicals; Analytical biochemistry -- Periodicals; Chemical laboratories -- Standards -- Periodicals; 543.1905
• DOI: 10.1039/c5ay03068d
• Languages: English
• ISSNs: 1759-9660

Abstract

Abstract : Immobilizing salicyl fluorone and hexadecyl pyridine bromide as chemosensors on a glutaraldehyde-coated eggshell membrane to design a "turn-off" fluorescent sensor for copper detection. Abstract : A sensitive and selective quantification of Cu 2+ by immobilizing a chromogenic agent on a chemosensor or biosensor has great significance in its scientific application. In this study, a kind of membrane sensor was applied to detect trace Cu 2+ by immobilizing salicyl fluorone (SAF) and hexadecyl pyridine bromide (CPB) on a glutaraldehyde (GA)-coated eggshell membrane (ESM). In the presence of CPB, the fluorescence emission peak of SAF at 545 nm was efficiently quenched by Cu 2+ through the formation of a ternary complex. The analysis conditions ( e.g. SAF concentration, CPB concentration and pH buffer solution) were investigated to establish an optimized method. The membrane sensor (SAF/CPB-GA-ESM) had a linear working range of 5–500 ng mL −1, while the detection limit for Cu 2+ was 0.5 ng mL −1 . Compared to the detection of Cu 2+ in aqueous media, the membrane sensor exhibited the advantages of low detection limits, high sensitivity and selectivity, as well as excellent stability. This fluorescent sensor was also successfully applied to the detection of Cu 2+ in water samples.