Comparison of the efficiency of 5 methods for fungal DNA extraction from crop debris and evaluation of its suitability for the amplification of Fusarium graminearum by PCR. (April 2016)

• Record Type: Journal Article
• Authors: Mourelos, Cecilia A.; Malbrán, Ismael; Megual Goméz, Diego; Balatti, Pedro A.; Ghiringhelli, Pablo D.; Lori, Gladys A.
• Is Part Of: Crop protection. Volume 82(2016)
• Page Start: 7
• Page End: 9
• Publication Date: 2016-04
• Subjects: Fusarium head blight -- Gibberella zeae -- Real-time PCR; Plants, Protection of -- Periodicals; 632.05
• DOI: 10.1016/j.cropro.2015.12.022
• Languages: English
• ISSNs: 0261-2194

Abstract

Abstract: Quantification of fungal DNA in crop debris by PCR could contribute to the development of strategies aimed at managing plant diseases. Five protocols were tested for its efficiency in the isolation of Fusarium graminearum DNA from crop debris. A CTAB-based method provided the best results (free of inhibitors) as confirmed by conventional and qPCR. Highlights: Optimization of a method for fungal DNA extraction from crop residues. Use of molecular tools for epidemiological studies. Quantification by real-time PCR of Fusarium graminearum DNA from crop debris.