Goldfish (Carassius auratus L.) as a model system to study the growth factors, receptors and transcription factors that govern myelopoiesis in fish. (May 2016)

• Record Type: Journal Article
• Authors: Katzenback, Barbara A.; Katakura, Fumihiko; Belosevic, Miodrag
• Is Part Of: Developmental and comparative immunology. Volume 58(2016)
• Page Start: 68
• Page End: 85
• Publication Date: 2016-05
• Subjects: Goldfish -- Myelopoiesis -- Macrophages -- Neutrophils -- Erythrocytes -- Thrombocytes -- Progenitor cells -- Transcription factors -- Growth factors; Immunology -- Periodicals; Developmental immunology -- Periodicals; 616.079
• DOI: 10.1016/j.dci.2015.10.024
• Languages: English
• ISSNs: 0145-305X

Abstract

Abstract: The process of myeloid cell development (myelopoiesis) in fish has mainly been studied in three cyprinid species: zebrafish ( Danio rerio ), ginbuna carp ( Carassius auratus langsdorfii ) and goldfish ( C. auratus, L.). Our studies on goldfish myelopoiesis have utilized in vitro generated primary kidney macrophage (PKM) cultures and isolated primary kidney neutrophils (PKNs) cultured overnight to study the process of macrophage (monopoiesis) and neutrophil (granulopoiesis) development and the key growth factors, receptors, and transcription factors that govern this process in vitro . The PKM culture system is unique in that all three subpopulations of macrophage development, namely progenitor cells, monocytes, and mature macrophages, are simultaneously present in culture unlike mammalian systems, allowing for the elucidation of the complex mixture of cytokines that regulate progressive and selective macrophage development from progenitor cells to fully functional mature macrophages in vitro . Furthermore, we have been able to extend our investigations to include the development of erythrocytes (erythropoiesis) and thrombocytes (thrombopoiesis) through studies focusing on the progenitor cell population isolated from the goldfish kidney. Herein, we review the in vitro goldfish model systems focusing on the characteristics of cell sub-populations, growth factors and their receptors, and transcription factors that regulate goldfish myelopoiesis. Highlights: Primary kidney macrophage cultures have been used to study the mechanisms of goldfish monopoiesis. Isolation of primary kidney neutrophils allowed the study of goldfish neutrophils and key molecules for their development. Colony-forming unit assays and key growth factors and receptors were used to study erythropoiesis and thrombopoiesis. Pathways of goldfish myelopoiesis require unique growth and transcription factors to instruct cell differentiation. Mechanisms of goldfish myelopoiesis are largely conserved across vertebrates, but unique differences also exist.