MAPKs and Hsc70 are critical to the protective effect of molecular hydrogen during the early phase of acute pancreatitis. (24th January 2016)

• Record Type: Journal Article
• Authors: Han, Bing; Zhou, Haoxin; Jia, Guang; Wang, Yongwei; Song, Zengfu; Wang, Gang; Pan, Shangha; Bai, Xuewei; Lv, Jiachen; Sun, Bei
• Is Part Of: FEBS journal. Volume 283:Number 4(2016)
• Page Start: 738
• Page End: 756
• Publication Date: 2016-01-24
• Subjects: acute pancreatitis -- Hsc70 -- MAPK pathway -- molecular hydrogen -- proteomics; Biochemistry -- Periodicals; Molecular biology -- Periodicals; Pathology, Molecular -- Periodicals; 572
• DOI: 10.1111/febs.13629
• Languages: English
• ISSNs: 1742-464X

Abstract

Abstract : Molecular hydrogen (H2 ) has been proven to be an effective agent that can cure multiple organ diseases by reducing oxidative stress. Although the protective effect of hydrogen on acute pancreatitis (AP) has been confirmed, its molecular mechanism is still unclear. In this article, we aimed to investigate the changes in pancreatic cell protein expression associated with the protective effect of H2 against AP and attempted to uncover the molecular mechanism underlying this process. A proteomic analysis identified 73 differentially expressed proteins and generated the protein–protein interaction networks of these proteins. The results triggered our interest in mitogen‐activated protein kinase (MAPK) and heat shock cognate 71 kDa protein (Hsc70). The subsequent in vitro experiments showed that H2 treatment inhibited the phosphorylation of extracellular signal‐regulated kinase (ERK), c‐jun N‐terminal kinase (JNK), and p38 MAPK, and activated NF‐κB and the expression of tumor necrosis factor α and interleukin‐1β, while simultaneously preventing the translocation of phospho‐ERK, phospho‐JNK, and phospho‐p38 from the cytoplasm to the nucleus. Furthermore, Hsc70 expression was upregulated by H2 administration. The animal experimental results were consistent with those of the in vitro experiments. In conclusion, H2 treatment can ameliorate the inflammatory response and reduce the expression of inflammatory mediators during the early phase of AP by inhibiting the MAPK pathways and increasing Hsc70 expression. Abstract : Molecular hydrogen (H2 ) has been shown to cure acute pancreatitis (AP). However, its molecular mechanism is unclear. We found that H2 treatment inhibited the activation and nuclear translocation of MAPKs signaling, and upregulated the Hsc70 expression. In conclusion, H2 treatment can ameliorate the inflammatory response during the early phase of AP by inhibiting the MAPK pathways and increasing Hsc70 expression.