Comparison of fetal cartilage‐derived progenitor cells isolated at different developmental stages in a rat model. (18th February 2016)

• Record Type: Journal Article
• Authors: Kim, Mijin; Kim, Jiyoung; Park, So Ra; Park, Do Young; Kim, Young Jick; Choi, Byung Hyune; Min, Byoung‐Hyun
• Is Part Of: Development growth and differentiation. Volume 58:Number 2(2016)
• Page Start: 167
• Page End: 179
• Publication Date: 2016-02-18
• Subjects: cartilage tissue -- cell therapy -- developmental stage -- differentiation -- fetal stem/progenitor cells; Embryology -- Periodicals; Developmental biology -- Periodicals; Growth -- Periodicals; 574.3
• DOI: 10.1111/dgd.12267
• Languages: English
• ISSNs: 0012-1592

Abstract

Abstract : Fetal cartilage‐derived progenitor cells (FCPCs) could be a useful cell source in cell‐based therapies for cartilage disorders. However, their characteristics can vary depending on the developmental stages. The aim of this study was to compare the characteristics of rat FCPCs from the hind limb on embryonic day 14 (E14), E16 and E20 regarding proliferation, pluripotency, and differentiation. Morphologically, rat fetal cartilage tissue showed an increase in cartilaginous differentiation features (Safranin‐O, type II collagen) and decrease in pluripotency marker (Sox2) in the order of E14, E16 and E20. E14 FCPCs showed significantly higher doubling time compared to E16 and E20 FCPCs. While the E14 FCPCs expressed pluripotent genes ( Sox2, Oct4, Nanog ), the E16 and E20 FCPCs expressed chondrogenic markers ( Sox9, Col2a1, Acan ). E20 FCPCs showed the highest ability to both chondrogenic and adipogenic differentiation and E14 FCPCs showed relatively better activity in osteogenic differentiation. Further analysis showed that E20 FCPCs expressed both adipogenic ( C/ebpß ) and osteogenic ( Runx2, Sp7, Taz ) transcription factors as well as chondrogenic transcription factors. Our results show an inverse relationship overall between the expression of pluripotency genes and that of chondrogenic and lineage‐specific genes in FCPCs under development. Due to its exceptional proliferation and chondrogenic differentiation ability, fetal cells from epiphyseal cartilage (E20 in rats) may be a suitable cell source for cartilage regeneration. Abstract : The aim of this study was to compare the characteristics of rat FCPCs from the hind limb on embryonic day 14 (E14), E16 and E20 regarding proliferation, pluripotency, and differentiation. Due to its exceptional proliferation and chondrogenic differentiation ability, fetal cells from epiphyseal cartilage may be a suitable cell source for cartilage regeneration.