The Pseudomonas aeruginosa AmrZ C‐terminal domain mediates tetramerization and is required for its activator and repressor functions. Issue 1 (21st December 2015)

• Record Type: Journal Article
• Authors: Xu, Binjie; Ju, Yue; Soukup, Randal J.; Ramsey, Deborah M.; Fishel, Richard; Wysocki, Vicki H.; Wozniak, Daniel J.
• Is Part Of: Environmental microbiology reports. Volume 8:Issue 1(2016:Feb.)
• Page Start: 85
• Page End: 90
• Publication Date: 2015-12-21
• Subjects: Microbial ecology -- Periodicals; Environmental Microbiology -- Periodicals; Electronic journals; 579.17
• DOI: 10.1111/1758-2229.12354
• Languages: English
• ISSNs: 1758-2229

Abstract

Summary: P seudomonas aeruginosa is an important bacterial opportunistic pathogen, presenting a significant threat towards individuals with underlying diseases such as cystic fibrosis. The transcription factor AmrZ regulates expression of multiple P . aeruginosa virulence factors. AmrZ belongs to the ribbon–helix–helix protein superfamily, in which many members function as dimers, yet others form higher order oligomers. In this study, four independent approaches were undertaken and demonstrated that the primary AmrZ form in solution is tetrameric. Deletion of the AmrZ C‐terminal domain leads to loss of tetramerization and reduced DNA binding to both activated and repressed target promoters. Additionally, the C‐terminal domain is essential for efficient AmrZ‐mediated activation and repression of its targets.