Enhanced and Differential Capture of Circulating Tumor Cells from Lung Cancer Patients by Microfluidic Assays Using Aptamer Cocktail. Issue 8 (13th January 2016)

• Record Type: Journal Article
• Authors: Zhao, Libo; Tang, Chuanhao; Xu, Li; Zhang, Zhen; Li, Xiaoyan; Hu, Haixu; Cheng, Si; Zhou, Wei; Huang, Mengfei; Fong, Anna; Liu, Bing; Tseng, Hsian‐Rong; Gao, Hongjun; Liu, Yi; Fang, Xiaohong
• Is Part Of: Small. Volume 12:Issue 8(2016)
• Page Start: 1072
• Page End: 1081
• Publication Date: 2016-01-13
• Subjects: aptamers -- cancer diagnosis -- circulating tumor cells -- rare cell enrichment -- tumor heterogeneity; Nanotechnology -- Periodicals; Nanoparticles -- Periodicals; Microtechnology -- Periodicals; 620.5
• DOI: 10.1002/smll.201503188
• Languages: English
• ISSNs: 1613-6810

Abstract

Abstract : Collecting circulating tumor cells (CTCs) shed from solid tumor through a minimally invasive approach provides an opportunity to solve a long‐standing oncology problem, the real‐time monitoring of tumor state and analysis of tumor heterogeneity. However, efficient capture and detection of CTCs with diverse phenotypes is still challenging. In this work, a microfluidic assay is developed using the rationally‐designed aptamer cocktails with synergistic effect. Enhanced and differential capture of CTCs for nonsmall cell lung cancer (NSCLC) patients is achieved. It is also demonstrated that the overall consideration of CTC counts obtained by multiple aptamer combinations can provide more comprehensive information in treatment monitoring. Abstract : Aptamer cocktails with a synergistic effect are obtained through a rational design. In the combined use of a silicon nanowire substrate embedded microfluidic chip, the enhanced and differential capture of circulating tumor cells (CTCs) is realized for nonsmall cell lung cancer patients. The potential of this assay in the characterization of CTC heterogeneity is discussed.