The establishment of efficient bioconversion, extraction, and isolation processes for the production of phyllodulcin, a potential high intensity sweetener, from sweet hydrangea leaves (Hydrangea macrophyllaThunbergii). Issue 2 (March 2016)

• Record Type: Journal Article
• Authors: Jung, Chang‐Hwan; Kim, Yang; Kim, Min‐Soo; Lee, Suyong; Yoo, Sang‐Ho
• Is Part Of: Phytochemical analysis. Volume 27:Issue 2(2016:Mar.)
• Page Start: 140
• Page End: 147
• Publication Date: 2016-03
• Subjects: Phyllodulcin -- Hydrangea macrophylla -- high intensity sweetener -- accelerated solvent extraction; Plants -- Analysis -- Periodicals; Plants -- chemistry -- Periodicals; 572.2
• DOI: 10.1002/pca.2609
• Languages: English
• ISSNs: 0958-0344

Abstract

Abstract: Introduction: Hydrangea leaf tea has been traditionally consumed in the far‐east Asian countries and is favoured for its distinct minty‐sweet taste. Phyllodulcin is identified as a key sweet‐tasting compound; it is 400–800 times sweeter than sucrose. However, its extraction has not been well‐documented. In an effort to optimise phyllodulcin production, pretreatment processes to accumulate phyllodulcin as a final metabolite in leaf tissue were studied, and an efficient process was established for the extraction and purification of phyllodulcin. Methods: Phyllodulcin was structurally identified using an LC/MS system. Hydrangea leaves were processed by either hand rolling or mechanical blending, by exposing them at different drying temperatures (25 and 70 °C), and even by inducing bioconversion in leaf tissue. The leaf powder was extracted with various solvents (methanol, ethanol, and water) by soaking at 25 °Cfor 12 h, ultrasonication at 35 °Cfor 1 h or accelerated solvent extraction (ASE). Extracts were purified with ion exchange resins and purified using preparative HPLC. Results: Traditional hand rolling and drying at 70 °Csignificantly increased phyllodulcin accumulation in the leaves. Meanwhile, more phyllodulcin was obtained from the leaves blended mechanically or converted enzymatically compared to traditionally processed ones ( P < 0.05). Methanol and ethanol were superior to water as extraction media, and the greatest phyllodulcin yields obtained by ASE, soaking and ultrasonication were 21.28, 21.20 and 19.33 mg/g, respectively, when methanol was used. Highly pure phyllodulcin powder was obtained with a yield of 2.12%. Conclusions: This promising result would be beneficial to the industrial utilisation of phyllodulcin as a potential high‐intensity sweetener . Copyright © 2016 John Wiley & Sons, Ltd. Abstract : The ways of pre‐treating hydrangea leaves were designed to maximally yield phyllodulcin via endogenous biocatalytic reaction. The greatest phyllodulcin was extracted using subcritical methanol with 3 cycles at 70°C for 5 min each. Phyllodulcin in the extract was isolated with a preparative HPLC after mixed‐bed ion exchanging step. Finally, over 99% purity of phyllodulcin powder was obtained with the yield of 2.12%. Industrial utilization of phyllodulcin as potential high‐intensity sweetener would be feasible due to this promising production process.