Expeditious dissolution dynamic nuclear polarization without glassing agents. (14th January 2016)

• Record Type: Journal Article
• Authors: Lama, Bimala; Collins, James H. P.; Downes, Daniel; Smith, Adam N.; Long, Joanna R.
• Is Part Of: NMR in biomedicine. Volume 29:Number 3(2016:Mar.)
• Page Start: 226
• Page End: 231
• Publication Date: 2016-01-14
• Subjects: DNP -- hyperpolarization -- glassing agents -- isopentane -- 13C MRS -- toxicity -- in vivo -- vitrified; Nuclear magnetic resonance -- Periodicals; Magnetic Resonance Spectroscopy -- Periodicals; 574
• DOI: 10.1002/nbm.3473
• Languages: English
• ISSNs: 0952-3480

Abstract

Abstract : The hyperpolarization of metabolic substrates at low temperature using dynamic nuclear polarization (DNP), followed by rapid dissolution and injection into an MRSI or NMR system, allows in vitro or in vivo observation and tracking of biochemical reactions and metabolites in real time. This article describes an elegant approach to sample preparation which is broadly applicable for the rapid polarization of aqueous small‐molecule substrate solutions and obviates the need for glassing agents. We demonstrate its utility for solutions of sodium acetate, pyruvate and butyrate. The polarization behavior of substrates prepared using rapid freezing without glassing agents enabled a 1.5–3‐fold time savings in polarization buildup, whilst removing the need for toxic glassing agents used as standard for dissolution DNP. The achievable polarization with fully aqueous substrate solutions was equal to that observed using standard approaches and glassing agents. Copyright © 2016 John Wiley & Sons, Ltd. Abstract : We describe an elegant sample preparation technique which is broadly applicable for the rapid polarization of small‐molecule substrates without glassing agents. The polarization behavior of substrates prepared using rapid vitrification without glassing agents enabled a 1.5–3‐fold time savings in polarization buildup, whilst removing the need for toxic glassing agents used as standard for dissolution DNP. The achievable polarization using vitrification and fully aqueous substrate solutions was equal to that observed using standard approaches and glassing agents.