Altered acetylation and succinylation profiles in Corynebacterium glutamicum in response to conditions inducing glutamate overproduction. Issue 1 (11th December 2015)

• Record Type: Journal Article
• Authors: Mizuno, Yuta; Nagano‐Shoji, Megumi; Kubo, Shosei; Kawamura, Yumi; Yoshida, Ayako; Kawasaki, Hisashi; Nishiyama, Makoto; Yoshida, Minoru; Kosono, Saori
• Is Part Of: MicrobiologyOpen. Volume 5:Issue 1(2016:Feb.)
• Page Start: 152
• Page End: 173
• Publication Date: 2015-12-11
• Subjects: 2‐oxoglutarate dehydrogenase complex -- corynebacterium glutamicum -- label‐free semi‐quantitative proteomic analysis -- l‐glutamate overproduction -- lysine acetylation -- lysine succinylation.; Microbiology -- Periodicals; 579
• DOI: 10.1002/mbo3.320
• Languages: English
• ISSNs: 2045-8827

Abstract

Abstract: The bacterium Corynebacterium glutamicum is utilized during industrial fermentation to produce amino acids such asl ‐glutamate. Duringl ‐glutamate fermentation, C . glutamicum changes the flux of central carbon metabolism to favorl ‐glutamate production, but the molecular mechanisms that explain these flux changes remain largely unknown. Here, we found that the profiles of two major lysine acyl modifications were significantly altered upon glutamate overproduction in C . glutamicum ; acetylation decreased, whereas succinylation increased. A label‐free semi‐quantitative proteomic analysis identified 604 acetylated proteins with 1328 unique acetylation sites and 288 succinylated proteins with 651 unique succinylation sites. Acetylation and succinylation targeted enzymes in central carbon metabolic pathways that are directly related to glutamate production, including the 2‐oxoglutarate dehydrogenase complex (ODHC), a key enzyme regulating glutamate overproduction. Structural mapping revealed that several critical lysine residues in the ODHC components were susceptible to acetylation and succinylation. Furthermore, induction of glutamate production was associated with changes in the extent of acetylation and succinylation of lysine, suggesting that these modifications may affect the activity of enzymes involved in glutamate production. Deletion of phosphotransacetylase decreased the extent of protein acetylation in nonproducing condition, suggesting that acetyl phosphate‐dependent acetylation is active in C . glutamicum . However, no effect was observed on the profiles of acetylation and succinylation in glutamate‐producing condition upon disruption of acetyl phosphate metabolism or deacetylase homologs. It was considered likely that the reduced acetylation in glutamate‐producing condition may reflect metabolic states where the flux through acid‐producing pathways is very low, and substrates for acetylation do not accumulate in the cell. Succinylation would occur more easily than acetylation in such conditions where the substrates for both acetylation and succinylation are limited. This is the first study investigating the acetylome and succinylome of C . glutamicum, and it provides new insight into the roles of acyl modifications in C . glutamicum biology. Abstract : The profiles of two major lysine acyl‐modifications, acetylation and succinylation, were significantly altered upon glutamate overproduction in C. glutamicum . A label‐free semi‐quantitative proteomic analysis identified 604 acetylated proteins with 1328 unique acetylation sites and 288 succinylated proteins with 651 unique succinylation sites.