Microfluidic synthesis of QD-encoded PEGDA microspheres for suspension assay. Issue 3 (17th December 2015)

• Record Type: Journal Article
• Authors: Liu, Huan; Qian, Xiang; Wu, Zhenjie; Yang, Rui; Sun, Shuqing; Ma, Hui
• Is Part Of: Journal of materials chemistry. Volume 4:Issue 3(2016)
• Page Start: 482
• Page End: 488
• Publication Date: 2015-12-17
• Subjects: Materials -- Periodicals; Chemistry, Analytic -- Periodicals; Biomedical materials -- Research -- Periodicals; 543.0284
• DOI: 10.1039/c5tb02209f
• Languages: English
• ISSNs: 2050-750X

Abstract

Abstract : A simple microfluidic device is designed to generate monodispersed QD-encoded PEGDA microbeads. PEGDA/PDA composite microspheres are prepared to easily couple protein on their surface. A sandwich immunoassay of rabbit IgG is performed to indicate that PDA on the bead surface facilitates efficient attachment of biomacromolecules. Abstract : Uniform and size-controllable QD-encoded poly(ethylene glycol) diacrylate (PEGDA) microbeads were produced using a microfluidic device followed by in situ photopolymerization. An S-shaped and gradually widening channel was designed to allow optimized UV exposure for photopolymerization and to prevent coalescence. The as-obtained PEGDA microbeads exhibited well-defined sphericity and excellent monodispersity with a coefficient of variation (CV) below 5%. The size varied from 7 μm to 120 μm and can be selectively achieved by simply adjusting the experimental parameters. The fluorescence performance of the QDs was well preserved without significant peak broadening or distortion. Seven barcode libraries were realized with bright fluorescence and distinguishing coding signals which could be conveniently decoded by a flow cytometer. Furthermore, a very facile strategy to conjugate biomolecules on the bead surfaces was developed using polydopamine (PDA). A sandwich immunoassay of rabbit IgG was performed and the applicability of the QD-encoded microbeads for suspension assay was demonstrated.