Preparation of high purity docosahexaenoic acid from microalgae oil in a packed bed reactor via two-step lipase-catalysed esterification. (March 2016)

• Record Type: Journal Article
• Authors: Lee, Eun-Ju; Lee, Moon Won; No, Da Som; Kim, Hee-Jin; Oh, Se-Wook; Kim, Yangha; Kim, In-Hwan
• Is Part Of: Journal of functional foods. Volume 21(2016)
• Page Start: 330
• Page End: 337
• Publication Date: 2016-03
• Subjects: Crypthecodinium cohnii -- Docosahexaenoic acid (DHA) -- Esterification -- Lipase -- Microalgae -- Rhizomucor miehei; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2015.12.020
• Languages: English
• ISSNs: 1756-4646

Abstract

Highlights: High purity docosahexaenoic acid (DHA) was prepared from microalgae oil. Lipase-catalysed esterification was employed for preparation of high purity DHA. The esterification was performed in packed bed reactor system. Finally, 100 wt% pure DHA was obtained from 42.5 wt% after two-step esterification. Abstract: Highly pure docosahexaenoic acid (DHA) was produced successfully in a packed bed reactor via the two-step lipase-catalysed esterification using the fatty acid from microalgae (from Crypthecodinium cohnii ) and ethanol as substrates. Lipozyme RM IM from Rhizomucor miehei was employed as a biocatalyst. In the first step, a temperature of 40 °C, a molar ratio of 1:4 (fatty acid to ethanol), and a water content of 0.6 wt% (based on the total substrate weight) were selected as optimum conditions. A maximum DHA concentration of ca. 90 wt% was achieved in the fatty acid fraction with an 89 wt% yield after 15 min. In the second step, the fatty acids from the first step were used as the substrate and a recursive reaction was conducted using identical optimized conditions. Following this second step, a DHA concentration of 100 wt% was achieved in conjunction with an 83.2 wt% yield after 25 min.