SHLA-G and sHLA-I levels in follicular fluid are not associated with successful implantation. (February 2016)

• Record Type: Journal Article
• Authors: Ouji-Sageshima, Noriko; Yuui, Katsuya; Nakanishi, Mari; Takeda, Nobuyoshi; Odawara, Yasushi; Yamashita, Masanori; Iwayama, Hiroshi; Awai, Keiko; Hashimoto, Hirotsugu; Geraghty, Daniel E.; Ishitani, Akiko; Hatake, Katsuhiko; Ito, Toshihiro
• Is Part Of: Journal of reproductive immunology. Volume 113(2016)
• Page Start: 16
• Page End: 21
• Publication Date: 2016-02
• Subjects: sHLA-G soluble HLA-G protein -- sHLA-G1 soluble HLA-G1 protein -- sHLA-I soluble HLA class I protein -- FFs follicular fluid samples -- ILT immunoglobulin-like transcript -- IVF in vitro fertilization; In vitro fertilization -- Follicular fluid -- HLA-G -- HLA class Ia; Reproduction -- Immunological aspects -- Periodicals; Immunology -- Periodicals; Allergy and Immunology -- Periodicals; Reproduction -- Periodicals; Reproduction -- Immunologie -- Périodiques; Immunologie -- Périodiques; Immunology; Reproduction -- Immunological aspects; Periodicals; Electronic journals; Electronic journals; 615.766
• DOI: 10.1016/j.jri.2015.10.001
• Languages: English
• ISSNs: 0165-0378

Abstract

Highlights: We examined the variation in sHLA-G levels among multiple FFs from one patient. The variation in sHLA-G levels was not related to successful implantation. The difference in sHLA-G levels was not related to successful implantation. We tested the presence of sHLA-G using immunoprecipitation (IP) and western blot (WB). The measurement of sHLA-G in FFs was not meaningful for selecting good oocytes. Abstract: In the field of in vitro fertilization (IVF), useful markers for the prediction of successful implantation for oocyte or embryo selection are essential. It has been reported that sHLA-G (sHLA-G1/HLA-G5) could be detected in the supernatant of the fertilized embryo and in follicular fluid samples (FFs), and that the presence of sHLA-G was related to successful implantation. If sHLA-G could be used as a marker of oocyte selection from multiple FFs, oocytes could be selected without physical contact, thus reducing the likelihood of damage. To investigate the potential for sHLA-G as a marker of oocyte selection from multiple FFs in one patient, protein levels of total protein, sHLA-G, and sHLA-I (sHLA-A, B, and C) were examined in FFs. The variation among multiple FFs in total protein level and sHLA-G level was not related to successful pregnancy. The average sHLA-I levels did not differ in the successful implantation and unsuccessful implantation groups, indicating that sHLA-I levels were not related to successful pregnancy. Furthermore, sHLA-G in FFs was not detected by western blotting, despite being detected by ELISA, while sHLA-I was detected by both ELISA and western blot. These data suggest that sHLA-G in FF might not be a useful marker for oocyte selection as measurements of sHLA-G were inconsistent and there was no association with successful pregnancy. Further, more rigorously tested ELISA systems for detecting sHLA-G in body fluids are necessary before the utility of sHLA-G for diagnosis can be established.