Cytotoxic effects induced by patulin, sterigmatocystin and beauvericin on CHO–K1 cells. (March 2016)

• Record Type: Journal Article
• Authors: Zouaoui, Nidhal; Mallebrera, Beatriz; Berrada, Houda; Abid-Essefi, Salwa; Bacha, Hassen; Ruiz, Maria-Jose
• Is Part Of: Food and chemical toxicology. Volume 89(2016)
• Page Start: 92
• Page End: 103
• Publication Date: 2016-03
• Subjects: Beauvericin -- Patulin -- Sterigmatocystin -- CHO–K1 cells -- Interaction effects; Toxicology -- Periodicals; Food poisoning -- Periodicals; Food Poisoning -- Periodicals; Toxicology -- Periodicals; Toxicologie -- Périodiques; Intoxications alimentaires -- Périodiques; Food poisoning; Toxicology; Periodicals; Electronic journals; 615.9
• DOI: 10.1016/j.fct.2016.01.010
• Languages: English
• ISSNs: 0278-6915

Abstract

Abstract: Mycotoxins are produced by different genera of fungi; mainly Aspergillus, Penicillium and Fusarium . The natural co-occurrence of beauvericin (BEA), patulin (PAT) and sterigmatocystin (STE) has been proved in feed and food commodities. This study investigates the cytotoxicity of individual and combined mycotoxins BEA, PAT and STE. The cytotoxicity on immortalized ovarian cells (CHO–K1) was evaluated using the MTT assay. After 24, 48 and 72 h, the IC50 values were 2.9 μM for PAT and ranged from 10.7 to 2.2 μM and from 25.0 to 12.5 μM for BEA and STE, respectively. Cytotoxic interactions were assayed by the isobologram method, which provides a combination index (CI) value as a quantitative measure of the three mycotoxin interaction's degree. Binary and tertiary combinations showed a dose dependent effect. At low fraction affected, mycotoxin combinations were synergetic; whereas, at higher fraction affected, the combinations showed additive effect. Our results indicate that the co-occurrence of low concentrations of mycotoxin in food may increase their toxic effects. Highlights: Individual BEA, PAT and STE are implicated in cytotoxicity effects. BEA, PAT and STE combinations increase toxicological effects on CHO–K1 cells. Synergistic effect was observed at low mycotoxins fraction affected. Combinations of mycotoxins at higher fraction affected showed additive effect.