Miscanthus × giganteus xylooligosaccharides: Purification and fermentation. (20th April 2016)

• Record Type: Journal Article
• Authors: Chen, Ming-Hsu; Bowman, Michael J.; Cotta, Michael A.; Dien, Bruce S.; Iten, Loren B.; Whitehead, Terence R.; Rausch, Kent D.; Tumbleson, M.E.; Singh, Vijay
• Is Part Of: Carbohydrate polymers. Volume 140(2016)
• Page Start: 96
• Page End: 103
• Publication Date: 2016-04-20
• Subjects: Miscanthus × giganteus -- Autohydrolysis -- Xylooligosaccharides -- Fermentation -- Acetic acid -- Lactic acid; d-Xylose (PubChem CID: 135191) -- Xylobiose (PubChem CID: 439538) -- Xylotriose (PubChem CID: 10201852) -- Xylotetraose (PubChem CID: 10230811) -- Xylopentaose (PubChem CID: 10146542) -- Xylohexaose (PubChem CID: 74539951) -- D-Glucose (PubChem CID: 5793) -- L-Arabinose (PubChem CID: 5460291) -- Acetic acid (PubChem CID: 176) -- Lactic acid (PubChem CID: 612); Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2015.12.052
• Languages: English
• ISSNs: 0144-8617

Abstract

Highlights: A XOS purification procedure was developed for miscanthus hydrolyzate. Purified M × G XOS had 89.1% (w/w) total substituted oligosaccharides. M × G XOS had 8.9% X2, 11.3% X3, 8.8% X4, 9.0% X5 and 5.8% X6. M × G XOS was compared with a commercial source of XOS in vitro . High utilization of X2, X3 and X4 were observed for two Bifidobacteria strains. Abstract: A procedure was developed to recover xylooligosaccharides (XOS) from Miscanthus × giganteus ( M × G ) hydrolyzate. M × G hydrolyzate was prepared using autohydrolysis, and XOS rich fractions were acquired using activated carbon adsorption and stepwise ethanol elution. The combined XOS fractions were purified using a series of ion exchange resin treatments. The end product, M × G XOS, had 89.1% (w/w) total substituted oligosaccharides (TSOS) composed of arabinose, glucose, xylose and acetyl group. Bifidobacterium adolescentis and Bifidobacterium catenulatum (health promoting bacteria) were cultured in vitro on M × G XOS and a commercial XOS source, which was used as a comparison. B. adolescentis grew to a higher cell density than B. catenulatum in both XOS cultures. Total xylose consumption for B. adolescentis was 84.1 and 84.8%, respectively for M × G and commercial XOS cultures; and for B. catenulatum was 76.6 and 73.6%, respectively. The xylobiose (X2), xylotriose (X3) and xylotetraose (X4) were almost utilized for both strains. Acetic and lactic acids were the major fermentation products of the XOS cultures.