New antimicrobial chitosan derivatives for wound dressing applications. (5th May 2016)

• Record Type: Journal Article
• Authors: Dragostin, Oana Maria; Samal, Sangram Keshari; Dash, Mamoni; Lupascu, Florentina; Pânzariu, Andreea; Tuchilus, Cristina; Ghetu, Nicolae; Danciu, Mihai; Dubruel, Peter; Pieptu, Dragos; Vasile, Cornelia; Tatia, Rodica; Profire, Lenuta
• Is Part Of: Carbohydrate polymers. Volume 141(2016)
• Page Start: 28
• Page End: 40
• Publication Date: 2016-05-05
• Subjects: Chitosan derivative -- Membrane -- Biomaterial -- Healing activity; ANOVA analysis of variance -- CS MMW chitosan with medium molecular weight -- DA degree of acetylation -- DS degree of substitution -- DMFA dimethylformamide -- FT-IR Fourier transform infrared spectroscopy -- 1H NMR proton nuclear magnetic resonance -- MSR membrane swelling ratio -- PBS phosphate buffered saline -- SEM scanning electron microscope -- TLC layer chromatography -- TPP sodium tripolyphosphate; Chitosan (PubChem CID: 21896651) -- Sulfadiazine (PubChem CID: 5215) -- Sulfamethoxydiazine (PubChem CID: 5326) -- Sulfamerazine (PubChem CID: 5325) -- Sulfadimethoxine (PubChem CID: 5323) -- Sulfisoxazole (PubChem CID:5344) -- Sulfamethoxazole (PubChem CID:5329) -- Chloroacetyl chloride (PubChem CID: 6577) -- Acetic acid (PubChem CID: 176) -- Sodium hydroxide (PubChem CID: 14798) -- Sodium tripolyphosphate (PubChem CID: 24455); Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2015.12.078
• Languages: English
• ISSNs: 0144-8617

Abstract

Highlights: New sulfoanamide-chitosan derivatives have been synthesized and characterized. Sulfoanamide-chitosan derivatives membranes have been prepared and characterized. Chitosan derivatives membranes have improved swelling and biodegradation rate. Chitosan-sulfadiazine membrane has good antimicrobial effect and healing properties. Abstract: Chitosan is a non-toxic, biocompatible, biodegradable natural cationic polymer known for its low imunogenicity, antimicrobial, antioxidant effects and wound-healing activity. To improve its therapeutic potential, new chitosan-sulfonamide derivatives have been designed to develop new wound dressing biomaterials. The structural, morphological and physico-chemical properties of synthesized chitosan derivatives were analyzed by FT-IR, 1 H NMR spectroscopy, scanning electron microscopy, swelling ability and porosity. Antimicrobial, in vivo testing and biodegradation behavior have been also performed. The chitosan derivative membranes showed improved swelling and biodegradation rate, which are important characteristics required for the wound healing process. The antimicrobial assay evidenced that chitosan-based sulfadiazine, sulfadimethoxine and sulfamethoxazole derivatives were the most active. The MTT assay showed that some of chitosan derivatives are nontoxic. Furthermore, the in vivo study on burn wound model induced in Wistar rats demonstrated an improved healing effect and enhanced epithelialization of chitosan-sulfonamide derivatives compared to neat chitosan. The obtained results strongly recommend the use of some of the newly developed chitosan derivatives as antimicrobial wound dressing biomaterials.