Nanoparticle-mediated transcriptional modification enhances neuronal differentiation of human neural stem cells following transplantation in rat brain. (April 2016)

• Record Type: Journal Article
• Authors: Li, Xiaowei; Tzeng, Stephany Y.; Liu, Xiaoyan; Tammia, Markus; Cheng, Yu-Hao; Rolfe, Andrew; Sun, Dong; Zhang, Ning; Green, Jordan J.; Wen, Xuejun; Mao, Hai-Quan
• Is Part Of: Biomaterials. Volume 84(2016)
• Page Start: 157
• Page End: 166
• Publication Date: 2016-04
• Subjects: Nanoparticles -- Transfection -- Neurogenin-2 -- Neuronal differentiation -- Traumatic brain injury; Biomedical materials -- Periodicals; Biocompatible Materials -- Periodicals; Biomatériaux -- Périodiques; 610.28
• DOI: 10.1016/j.biomaterials.2016.01.037
• Languages: English
• ISSNs: 0142-9612

Abstract

Abstract: Strategies to enhance survival and direct the differentiation of stem cells in vivo following transplantation in tissue repair site are critical to realizing the potential of stem cell-based therapies. Here we demonstrated an effective approach to promote neuronal differentiation and maturation of human fetal tissue-derived neural stem cells (hNSCs) in a brain lesion site of a rat traumatic brain injury model using biodegradable nanoparticle-mediated transfection method to deliver key transcriptional factor neurogenin-2 to hNSCs when transplanted with a tailored hyaluronic acid (HA) hydrogel, generating larger number of more mature neurons engrafted to the host brain tissue than non-transfected cells. The nanoparticle-mediated transcription activation method together with an HA hydrogel delivery matrix provides a translatable approach for stem cell-based regenerative therapy.