Bagasse hydrolyzates from Agave tequilana as substrates for succinic acid production by Actinobacillus succinogenes in batch and repeated batch reactor. (April 2016)

• Record Type: Journal Article
• Authors: Corona-González, Rosa Isela; Varela-Almanza, Karla María; Arriola-Guevara, Enrique; Martínez-Gómez, Álvaro de Jesús; Pelayo-Ortiz, Carlos; Toriz, Guillermo
• Is Part Of: Bioresource technology. Volume 205(2016)
• Page Start: 15
• Page End: 23
• Publication Date: 2016-04
• Subjects: Actinobacillus succinogenes -- Acid hydrolyzates -- Enzymatic hydrolyzates -- Succinic acid -- Repeated batch fermentations; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2015.12.081
• Languages: English
• ISSNs: 0960-8524

Abstract

Highlights: Lignocellulosic residues can be used as a source of hydrolyzates for fermentation. Enzymatic hydrolyzates were free of furfural and hydroxymethylfurfural. Production of succinic acid was faster by using enzymatic hydrolyzates. 33.6 g/L succinic acid was obtained from 87.2 g/L fermentable sugars by repeated batch. Immobilization of cells reduced significantly the time of fermentation. Abstract: The aim of this work was to obtain fermentable sugars by enzymatic or acid hydrolyses of Agave tequilana Weber bagasse in order to produce succinic acid with Actinobacillus succinogenes . Hydrolyses were carried out with mineral acids (sulfuric and hydrochloric acids) or a commercial cellulolytic enzyme, and were optimized statistically by a response surface methodology, having as factors the concentration of acid/enzyme and time of hydrolysis. The concentration of sugars obtained at optimal conditions for each hydrolysis were 21.7, 22.4 y 19.8 g/L for H2 SO4, HCl and the enzymatic preparation respectively. Concerning succinic acid production, the enzymatic hydrolyzates resulted in the highest yield (0.446 g/g) and productivity (0.57 g/L h) using A. succinogenes in a batch reactor system. Repeated batch fermentation with immobilized A. succinogenes in agar and with the enzymatic hydrolyzates resulted in a maximum concentration of succinic acid of 33.6 g/L from 87.2 g/L monosaccharides after 5 cycles in 40 h, obtaining a productivity of 1.32 g/Lh.