Biotransformation kinetics of pharmaceutical and industrial micropollutants in groundwaters by a laccase cocktail from Pycnoporus sanguineus CS43 fungi. (March 2016)

• Record Type: Journal Article
• Authors: Rodríguez-Delgado, Melissa; Orona-Navar, Carolina; García-Morales, Raúl; Hernandez-Luna, Carlos; Parra, Roberto; Mahlknecht, Jürgen; Ornelas-Soto, Nancy
• Is Part Of: International biodeterioration & biodegradation. Volume 108(2016)
• Page Start: 34
• Page End: 41
• Publication Date: 2016-03
• Subjects: Laccase -- Pycnoporus sanguineus -- Micropollutants -- Biotransformation -- Groundwater; Biodegradation -- Periodicals; Bioremediation -- Periodicals; Biodegradation -- Periodicals; Biodégradation -- Périodiques; Biorestauration -- Périodiques; Electronic journals; 620.11223
• DOI: 10.1016/j.ibiod.2015.12.003
• Languages: English
• ISSNs: 0964-8305

Abstract

Abstract: In this work, the biocatalytic ability of laccases from filtered culture supernatant of Pycnoporus sanguineus was evaluated without mediators and under mild reaction conditions. This 100 U L −1 laccase cocktail removed 50% Diclofenac, 97% β-Naphthol and 71% 2, 4 Dichlorophenol within 8 h of reaction and 78% for 5, 7-Diiodo-8-hydroxyquinoline within 3.5 h; at initial concentrations of 10 mg L −1 and at 25 °C. Furthermore, this enzyme cocktail also removed in excess of 53% all tested compounds in a real groundwater sample from northwestern Mexico. In comparison with purified laccases, the use of cocktail offers operational advantages since additional purification steps can be avoided. Highlights: Bioconversion by Pycnoporus sanguineus laccase, avoiding the use of mediators and under mild conditions. Laccase showed high removal for all micropollutants tested in synthetic and real complex matrices. Biotransformation analysis in groundwaters matrices using P. sanguineus laccases. A decrease in bioconversion rates were observed cause by interaction of the ions in groundwater. Bioconversion of the antibiotic 5, 7-Diiodo-8-hydroxyquinoline by laccase, for first time.