Elemental bio-imaging using laser ablation-triple quadrupole-ICP-MS. Issue 1 (17th September 2015)

• Record Type: Journal Article
• Authors: Bishop, David P.; Clases, David; Fryer, Fred; Williams, Elizabeth; Wilkins, Simon; Hare, Dominic J.; Cole, Nerida; Karst, Uwe; Doble, Philip A.
• Is Part Of: Journal of analytical atomic spectrometry. Volume 31:Issue 1(2016:Jan.)
• Page Start: 197
• Page End: 202
• Publication Date: 2015-09-17
• Subjects: Atomic spectra -- Periodicals; Atomic absorption spectroscopy -- Periodicals; 543.0858
• DOI: 10.1039/c5ja00293a
• Languages: English
• ISSNs: 0267-9477

Abstract

Abstract : Elemental bio-imaging using the technological advances of ICP-QQQ-MS. Abstract : Elemental bio-imaging (EBI) of trace metal distributions in tissue sections is typically performed by laser ablation-single quadrupole-inductively coupled plasma-mass spectrometry (LA-ICP-SQ-MS) in which the sensitivity of biologically relevant elements such as Fe may be inhibited by polyatomic interferences. The triple quadrupole ICP-MS (ICP-QQQ-MS) is designed to reduce interferences and was examined for its potential application to EBI. Three tune modes; a no-gas, an H2, and an O2 /H2 tune were developed and investigated for their suitability. All three performed well with high signal to noise ratios and low limits of detection. The O2 /H2 tune was applied to a prostate cancer biopsy sample, with high levels of Zn found in the area correlating with the cancer. The oxygen mass shift was applied to image P and Se in a mouse brain. The unique focussing properties of the ICP-QQQ-MS and the ability to use mixed cell gases provided sensitive analyses of all measured isotopes with the O2 /H2 tune, including those unaffected by mass shift reactions.