Conversion of a non-heme iron-dependent sulfoxide synthase into a thiol dioxygenase by a single point mutation. Issue 9 (18th December 2015) - Goncharenko, Kristina V.
Abstract : EgtB from Mycobacterium thermoresistibile catalyzes O2 -dependent sulfur–carbon bond formation between the side chains of N α-trimethyl histidine and γ-glutamyl cysteine as a central step in ergothioneine biosynthesis. Abstract : EgtB from Mycobacterium thermoresistibile catalyzes O2 -dependent sulfur–carbon bond formation between the side chains of N α-trimethyl histidine and γ-glutamyl cysteine as a central step in ergothioneine biosynthesis. A single point mutation converts this enzyme into a γ-glutamyl cysteine dioxygenase with an efficiency that rivals naturally evolved thiol dioxygenases.