Improved oxidation of aromatic and aliphatic hydrocarbons using rate enhancing variants of P450Bm3 in combination with decoy molecules. Issue 5 (23rd November 2015)

• Record Type: Journal Article
• Authors: Munday, Samuel D.; Shoji, Osami; Watanabe, Yoshihito; Wong, Luet-Lok; Bell, Stephen G.
• Is Part Of: Chemical communications. Volume 52:Issue 5(2016)
• Page Start: 1036
• Page End: 1039
• Publication Date: 2015-11-23
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/c5cc09247g
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : The addition of perfluorinated fatty acids to the rate accelerating KT2 mutant of P450Bm3 resulted in the highly active oxidation of cyclohexane and benzenes whilst maintaining the product selectivity. Abstract : Enzyme performance can be improved using decoy molecules or engineered variants to accelerate the activity without affecting selectivity. Here we combine a rate accelerator variant of cytochrome P450Bm3 with decoy molecules to enhance the oxidation activity of a range of small organic molecules. This combined approach offers superior biocatalytic efficiency without modifying the product distribution.