Design and synthesis of fluorescence-labeled nucleotide with a cleavable azo linker for DNA sequencing. Issue 5 (20th November 2015)

• Record Type: Journal Article
• Authors: Tan, Lianjiang; Liu, Yazhi; Yang, Qinglai; Li, Xiaowei; Wu, Xin-Yan; Gong, Bing; Shen, Yu-Mei; Shao, Zhifeng
• Is Part Of: Chemical communications. Volume 52:Issue 5(2016)
• Page Start: 954
• Page End: 957
• Publication Date: 2015-11-20
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/c5cc09131d
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : A 3′-OH unblocked nucleotide analogue with cleavable azo linker was synthesized as a potential reversible terminator for DNA sequencing. Abstract : A cleavable azo linker was synthesized and reacted with 5-(6)-carboxytetramethyl rhodamine succinimidyl ester, followed by further reactions with di( N -succinimidyl) carbonate and 5-(3-amino-1-propynyl)-2′-deoxyuridine 5′-triphosphate [dUTP(AP3 )] to obtain the terminal product dUTP-azo linker-TAMRA as a potential reversible terminator for DNA sequencing by synthesis with no need for 3′-OH blocking.