A simple molecular beacon with duplex-specific nuclease amplification for detection of microRNA. Issue 3 (21st December 2015)

• Record Type: Journal Article
• Authors: Li, Yingcun; Zhang, Jiangyan; Zhao, Jingjing; Zhao, Likun; Cheng, Yongqiang; Li, Zhengping
• Is Part Of: Analyst. Volume 141:Issue 3(2016)
• Page Start: 1071
• Page End: 1076
• Publication Date: 2015-12-21
• Subjects: Chemistry, Analytic -- Periodicals; 543
• DOI: 10.1039/c5an02312b
• Languages: English
• ISSNs: 0003-2654

Abstract

Abstract : A target recycling amplification method based on a simple molecular beacon and duplex-specific nuclease has been developed for microRNA detection. Abstract : MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene activity, promoting or inhibiting cell proliferation, migration and apoptosis. Abnormal expression of miRNAs is associated with many diseases. Therefore, it is essential to establish a simple, rapid and sensitive miRNA detection method. In this paper, based on a simple molecular beacon (MB) and duplex-specific nuclease (DSN), we developed a target recycling amplification method for miRNA detection. By controlling the number of stem bases to 5, the MB probe used in this method can be prevented from hydrolysis by DSN without special modification. This assay is direct and simple to quantitatively detect miRNA with high sensitivity and specificity. The MB probe design provides a new strategy for nuclease-based amplification reaction.