A simple integrated microfluidic device for the multiplexed fluorescence-free detection of Salmonella enterica. Issue 3 (14th December 2015)

• Record Type: Journal Article
• Authors: Strachan, Briony C.; Sloane, Hillary S.; Houpt, Eric; Lee, Jacob C.; Miranian, Daniel C.; Li, Jingyi; Nelson, Daniel A.; Landers, James P.
• Is Part Of: Analyst. Volume 141:Issue 3(2016)
• Page Start: 947
• Page End: 955
• Publication Date: 2015-12-14
• Subjects: Chemistry, Analytic -- Periodicals; 543
• DOI: 10.1039/c5an01969a
• Languages: English
• ISSNs: 0003-2654

Abstract

Abstract : The integration of PCR with a particle-based method for visual DNA detection allows sequence-specific detection of bacterial targets in less than 35 minutes. Abstract : Rapid, inexpensive and simplistic nucleic acid testing (NAT) is pivotal in delivering biotechnology solutions at the point-of-care (POC). We present a poly(methylmethacrylate) (PMMA) microdevice where on-board infrared-mediated PCR amplification is seamlessly integrated with a particle-based, visual DNA detection for specific detection of bacterial targets in less than 35 minutes. Fluidic control is achieved using a capillary burst valve laser-ablated in a novel manner to confine the PCR reagents to a chamber during thermal cycling, and a manual torque-actuated pressure system to mobilize the fluid from the PCR chamber to the detection reservoir containing oligonucleotide-adducted magnetic particles. Interaction of amplified products specific to the target organism with the beads in a rotating magnetic field allows for near instantaneous (<30 s) detection based on hybridization-induced aggregation (HIA) of the particles and simple optical analysis. The integration of PCR with this rapid, sequence-specific DNA detection method on a single microdevice presents the possibility of creating POC NAT systems that are low cost, easy-to-use, and involve minimal external hardware.