Alkyl detection facilitated by a DNA conjugate with an α-hemolysin nanopore. Issue 1 (21st December 2015)

• Record Type: Journal Article
• Authors: Hu, Zheng-Li; Cao, Chan; Wang, Hui-Feng; Yan, Bing-Yong
• Is Part Of: RSC advances. Volume 6:Issue 1(2016)
• Page Start: 105
• Page End: 108
• Publication Date: 2015-12-21
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/c5ra20425a
• Languages: English
• ISSNs: 2046-2069

Abstract

Abstract : A novel α-hemolysin nanopore (α-HL) based strategy for the detection of an alkyl linker at the single-molecule level. Abstract : Herein an α-hemolysin nanopore was utilized to detect a DNA conjugate, where a propyl linker was attached to the 20-mer oligonucleotides at each end of an individual strand. The studies showed the alkyl linker produced a clear current change when translocating through the protein pore, which could be used to sense and identify small organic molecule at the single-molecule level.