Fabrication of a dendrimer-modified boronate affinity material for online selective enrichment of cis-diol-containing compounds and its application in determination of nucleosides in urine. Issue 128 (14th December 2015)

• Record Type: Journal Article
• Authors: Gao, Li; Du, Jin; Wang, Chaozhan; Wei, Yinmao
• Is Part Of: RSC advances. Volume 5:Issue 128(2015)
• Page Start: 106161
• Page End: 106170
• Publication Date: 2015-12-14
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/c5ra18443f
• Languages: English
• ISSNs: 2046-2069

Abstract

Abstract : A high binding capacity dendrimer-modified boronate affinity material (SiO2 @dBA) was synthesized and coupled with large-volume injection/online column-switching solid phase extraction to facilitate the determination process of cis -diols. Abstract : Boronate adsorbents have been widely used in the extraction of cis -diol-containing molecules, but most do not have efficient capacity due to limited binding sites on their surface. In this work, a high binding capacity dendrimer-modified boronate affinity material (SiO2 @dBA) was synthesized via introducing tris(2-aminoethyl)amine as branching points and using poly(amidoamine) as the main dendrimeric scaffold before modification by boronate groups. The high density of amino groups on the dendrimer supplied a large number of binding sites for modifying boronate groups. Thus the adsorption capacity (676.8 μmol g −1 for catechol, 771.3 μmol g −1 for dopamine, 770.0 μmol g −1 for adrenaline) of SiO2 @dBA was greatly improved. Moreover, when coupled with large-volume injection and online column-switching solid phase extraction, SiO2 @dBA was able to capture cis -diols from 10 000-fold interference and enrichment factors reached up to 497–514, which was 26- to 51-fold higher than those of analogous non-dendrimer materials. Especially, the proposed method exhibited a striking low limit of detection, 0.24 ng mL −1 for cytidine, 0.52 ng mL −1 for uridine, 0.37 ng mL −1 for guanosine, 0.67 ng mL −1 for adenosine. Finally, the method was successfully applied to online determination of trace nucleosides in healthy human urine. In conclusion, the prepared adsorbent has potential to effectively enrich a large range of trace cis -diol substances in real samples.