Analysis of sphingomyelin in plasma membrane at single cells using luminol electrochemiluminescence. Issue 12 (25th January 2016)

• Record Type: Journal Article
• Authors: Chen, Yao; Liu, Yanan; Xia, Juan; Liu, Jing; Jiang, Dechen; Jiang, Depeng
• Is Part Of: RSC advances. Volume 6:Issue 12(2016)
• Page Start: 9518
• Page End: 9521
• Publication Date: 2016-01-25
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/c5ra24275d
• Languages: English
• ISSNs: 2046-2069

Abstract

Abstract : Luminol electrochemiluminescence was applied to detect sphingomyelin in plasma membrane at single cells that exhibited the high cellular heterogeneity. Abstract : Sphingomyelin in plasma membrane at single cells was analyzed using luminol electrochemiluminescence, which involved serial reactions to generate hydrogen peroxide from membrane sphingomyelin for luminescence detection. A large deviation of the luminescence ratio before and after the introduction of the enzyme mixture was obtained from single cell analysis indicating the high cellular heterogeneity.