Development of an eco-friendly immunochromatographic test strip and its application in detecting Hg2+ without chelators. Issue 11 (21st January 2016)

• Record Type: Journal Article
• Authors: Wang, Suyan; Wang, Limin; Chen, Hongfu; Wang, Yulong; Cai, Jia; Yang, Mingming; Liu, Fengquan
• Is Part Of: RSC advances. Volume 6:Issue 11(2016)
• Page Start: 8729
• Page End: 8735
• Publication Date: 2016-01-21
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/c5ra22752f
• Languages: English
• ISSNs: 2046-2069

Abstract

Abstract : The schematic diagram of an eco-friendly immunochromatographic test strip (EFITS) and a competitive immunoreaction on the EFITS. Abstract : Here, a specific anti-Hg 2+ monoclonal antibody (mAb) was generated and an eco-friendly immunochromatographic test strip (EFITS) based on a mAb-nanogold probe for rapid and specific detection of Hg 2+ in water was developed. In the method, the conjugation of Hg–MNA (6-mercaptonicotinic acid)–BSA (bovine serum albumin) was synthesized as an immunogen, the conjugation of MNA–OVA (ovalbumin) was synthesized and selected as a coating antigen. The specific anti-Hg 2+ mAb from BALB/C female mice was screened based on a competitive immunoassay. The coating antigen and goat anti-mouse IgG antibody were coated on a nitrocellulose membrane (NC membrane) as a test line and a control line, respectively. The anti-Hg 2+ mAb-nanogold probe was applied to the conjugate pad. Hg 2+ competes with OVA–MNA for the mAb-nanogold probe causing a color change on the test line corresponding to the Hg 2+ content. Thus, we can distinguish the subtle differences through a strip reader. The resulting EFITS is able to detect Hg 2+ with LOD of 0.4 ng mL −1 at 9 min by quantitative analysis. EFITS demonstrated here is eco-friendly (without Hg 2+ on the strip), capable of rapid detection, and does not require chelators.