A novel aptamer-mediated CuInS2 quantum dots@graphene oxide nanocomposites-based fluorescence "turn off–on" nanosensor for highly sensitive and selective detection of kanamycin. Issue 12 (26th January 2016)

• Record Type: Journal Article
• Authors: Liu, Ziping; Tian, Chengshuo; Lu, Lehui; Su, Xingguang
• Is Part Of: RSC advances. Volume 6:Issue 12(2016)
• Page Start: 10205
• Page End: 10214
• Publication Date: 2016-01-26
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/c5ra22753d
• Languages: English
• ISSNs: 2046-2069

Abstract

Abstract : A novel aptamer-mediated fluorescence "turn off–on" nanosensor for highly sensitive and selective detection of kanamycin using CuInS2 quantum dots@graphene oxide. Abstract : In this paper, we designed a novel near-infrared aptamer-mediated fluorescence "turn off–on" nanosensor for highly sensitive and selective detection of kanamycin based on CuInS2 quantum dots (QDs)@graphene oxide (GO) nanocomposites. The carboxy groups on the surface of CuInS2 QDs (modified with mercaptopropionic acid) were conjugated with amino terminal kanamycin-binding Ky2 aptamer to form the Ky2-CuInS2 QDs in the presence of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and N -hydroxysuccinimide. Then, the Ky2-CuInS2 QDs were facilely immobilized on the surface of GO through π–π stacking interaction between the nucleobases and GO, which caused the fluorescence of Ky2-CuInS2 QDs "turned off". In the presence of kanamycin, the Ky2-CuInS2 QDs desorb from the surface of GO and bind to kanamycin with high affinity and specificity. As a result, the quenched fluorescence "turned on". Under the optimum conditions, there was a good linear relationship between I / I 0 ( I and I 0 were the fluorescence intensity of Ky2-CuInS2 QDs@GO in the presence and absence of kanamycin, respectively) and kanamycin concentration in the range of 0.3–45 nmol L −1 (0.174–26.1 μg L −1 ), with the detection limit of 0.12 nmol L −1 (0.070 μg L −1 ). The present nanosensor was utilized to detect kanamycin in the human serum, urine and milk samples with satisfactory results.