Effect of DDT exposure on lipids and energy balance in obese Sprague-Dawley rats before and after weight loss. (2015)

• Record Type: Journal Article
• Authors: Ishikawa, Tomoko; Graham, James L.; Stanhope, Kimber L.; Havel, Peter J.; La Merrill, Michele A.
• Is Part Of: Toxicology reports. Volume 2(2015)
• Page Start: 990
• Page End: 995
• Publication Date: 2015
• Subjects: DDT dichlorodiphenyltrichloroethane -- DDE dichlorodiphenyldichloroethylene -- HFD high fat diet -- CR caloric restriction -- SD Sprague Dawley -- NEFA non esterified fatty acid -- T2DM type 2 diabetes mellitus -- CVD cardiovascular disease -- TG triglyceride -- OLTT oral lipid tolerance test -- TSH thyroid-stimulating hormone -- T4 thyroxine -- T3 triiodothyronine; DDE -- DDT -- Food efficiency -- Dyslipidemia -- Thermoregulation -- Thyroid hormone; Toxicology -- Periodicals; Clinical toxicology -- Periodicals; Drug-Related Side Effects and Adverse Reactions; Hazardous Substances; Poisoning; Toxicology; Electronic journals; Periodicals; Periodicals; 571.9505
• DOI: 10.1016/j.toxrep.2015.07.005
• Languages: English
• ISSNs: 2214-7500

Abstract

Abstract: Dichlorodiphenyltrichloroethane (DDT) and its metabolites accumulate in adipose tissue through dietary exposure, and have been proposed to contribute to the development of abdominal obesity, insulin resistance and dyslipidemia. Toxicity may also result when DDT and its metabolites are released from adipose tissue into the bloodstream as a result of rapid weight loss. We hypothesized that DDT-exposed rats fed a high fat diet (HFD) followed by 60% calorie restriction would have an adverse metabolic response to rapid weight loss. To test this, we exposed obese Sprague-Dawley (SD) rats to DDT and a HFD over one month followed by 60% calorie restricted diet for two weeks, and examined metabolic parameters throughout the study. During the HFD feeding period, DDT-exposed rats had significantly elevated postprandial non-esterified fatty acids (NEFAs) and decreased body temperature compared with control rats. During calorie restriction, DDT-exposed rats had lowered food efficiency (weight gained/calories consumed), body temperature, and circulating TSH. Our findings suggest that exposure to DDT may impairs metabolic substrate utilization in rats during dynamic periods of weight gain and weight loss.