A supersandwich fluorescence in situ hybridization strategy for highly sensitive and selective mRNA imaging in tumor cells. Issue 2 (2nd November 2015)

• Record Type: Journal Article
• Authors: Huang, Jin; Wang, He; Yang, Xiaohai; Yang, Yanjing; Quan, Ke; Ying, Le; Xie, Nuli; Ou, Min; Wang, Kemin
• Is Part Of: Chemical communications. Volume 52:Issue 2(2016)
• Page Start: 370
• Page End: 373
• Publication Date: 2015-11-02
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/c5cc08503a
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : This strategy uses two fluorophore-labeled signal probes to generate a supersandwich product, which in turn generates numerous signal probes located at the target mRNA position, resulting in the in situ fluorescence signal amplification. Abstract : We report a supersandwich fluorescence in situ hybridization (SFISH) strategy for highly sensitive and selective in situ visualization of mRNA expression patterns at the single-cell level. This strategy uses two fluorophore-labeled signal probes to generate a supersandwich product, which in turn generates numerous signal probes located at the target mRNA position, resulting in the in situ fluorescence signal amplification.