Determination of diethylstilbestrol in seawater by molecularly imprinted solid-phase extraction coupled with high-performance liquid chromatography. Issue 1 (15th January 2016) - He, Xiuping
Abstract: An effective and highly selective molecularly imprinted material was prepared by suspension polymerization for the isolation and pre-concentration of synthetic estrogen diethylstilbestrol (DES) in seawater. The obtained MIPMs were proved to have more uniform size and porous structure, with maximum adsorption capacity of 8.43 mg g − 1 almost two times more than NIPMs (4.43 mg g − 1 ). The MIPMs showed no significant deterioration of the adsorption capacity after five rounds of regeneration. An off-line molecularly imprinted solid phase extraction (MISPE) method followed by HPLC–DAD was proposed for the detection of DES in seawater, and recoveries were satisfactorily higher than 77%. Four seawater samples in aquaculture area were analyzed and 0.61 ng mL − 1 DES was detected in one sample. The result demonstrated that this method can be used for the rapid separation and clean up of trace residual of DES in seawater. Graphical abstract: Highlights: The diethylstilbestrol molecularly imprinted polymer microspheres (DES-MIPMs) were prepared. The characteristics, binding capacity and recyclability of the MIPMs were studied. An off-line method using MIPMs as solid phase extraction sorbent for DES was developed. Four seawater samples were determined in two aquaculture areas. The DES concentration in one sample was detected.