Enhanced biodegradation of pyrene and indeno(1, 2, 3-cd)pyrene using bacteria immobilized in cinder beads in estuarine wetlands. Issue 1 (15th January 2016)

• Record Type: Journal Article
• Authors: Huang, Ru-ying; Tian, Wei-jun; Liu, Qing; Yu, Hui-bo; Jin, Xin; Zhao, Yang-guo; Zhou, Yu-hang; Feng, Gong
• Is Part Of: Marine pollution bulletin. Volume 102:Issue 1(2016:Jan.)
• Page Start: 128
• Page End: 133
• Publication Date: 2016-01-15
• Subjects: Pyrene -- Indeno(1, 2, 3-cd)pyrene -- Immobilized -- Cinder beads -- Biodegradation; Marine pollution -- Periodicals; Marine Biology -- Periodicals; Water Pollution -- Periodicals; Mer -- Pollution -- Périodiques; Publications périodiques; Pollution des mers; Lutte antipollution; Electronic journals; 363.7394
• DOI: 10.1016/j.marpolbul.2015.11.044
• Languages: English
• ISSNs: 0025-326X

Abstract

ABSTRACT: Two strains ( Pseudomonas taiwanensis PYR1 and Acinetobacter baumannii INP1) were isolated from PAH-contaminated Liaohe estuarine wetland using enrichment. The cells of PYR1 and INP1 were immobilized in cinder beads for pyrene and indeno(1, 2, 3-cd)pyrene biodegradation in wetland. Biodegradation of pyrene and indeno(1, 2, 3-cd)pyrene in soils from wetland was carried out in pots using free cells as well as those immobilized in cinder beads to ascertain the role of bioaugmentation. Supported by the cinder beads, the immobilized cells degraded 70.7% and 80.9% of pyrene and indeno(1, 2, 3-cd)pyrene respectively after 30 days. While the free cells degraded only 58.2% and 55.3%. Additionally, microbial analysis with high-throughput sequencing revealed the changes of microbial communities in soil without and with cinder beads immobilized with strains. The result indicated that Gammaproteobacteria were dominant PAH-degrading groups during bioaugmentation. This effective approach can be used to treat other PAH-contaminated wetlands by immobilizing different species of bacteria in cinder beads. Highlights: Two strains ( Pseudomonas putida PYR1 and Acinetobacter baumannii INP1) were isolated from PAH-contaminated Liaohe estuarine wetlands, China. The method to prepare cinder beads with immobilized bacteria was provided. The ability of immobilized bacteria to degrade pyrene and indeno(1, 2, 3-cd)pyrene was evaluated. Changes of microbial communities in soils during bioaugmentation were analyzed.