Structural elucidation and protective role of a polysaccharide from Sargassum fusiforme on ameliorating learning and memory deficiencies in mice. (30th March 2016)

• Record Type: Journal Article
• Authors: Hu, Pei; Li, Zhixiong; Chen, Mingcang; Sun, Zhaolin; Ling, Yun; Jiang, Jian; Huang, Chenggang
• Is Part Of: Carbohydrate polymers. Volume 139(2016)
• Page Start: 150
• Page End: 158
• Publication Date: 2016-03-30
• Subjects: AD Alzheimer's disease -- SFPS65 Sargassum fusiforme polysaccharide 65 -- LC–ESI-Q-TOF-MS high-performance liquid chromatography coupled with electrospray ionization quadruple time-of-flight tandem mass spectrometry -- HPGPC high-performance gel permeation chromatography -- IR infrared spectrum -- NMR nuclear magnetic resonance -- CMC-Na sodium carboxymethylcellulose; Fucoidan -- ESI-Q-TOF-MS -- Chemical structure -- Sargassum fusiforme -- Alzheimer's disease; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2015.12.019
• Languages: English
• ISSNs: 0144-8617

Abstract

Highlights: A new fucoidan was isolated from Sargassum fusiforme . The predicted structure of fucoidan was elucidated by chemical and spectral methods. The fucoidan exhibited a good effect on improving cognitive dysfunction of the mice. The fucoidan was the main medicinal ingredients on treatment of Alzheimer's disease. Abstract: A fucoidan, Sargassum fusiforme polysaccharide 65 (SFPS65) A, was isolated from a brown alga ( S. fusiforme ). SFPS65A had an estimated molecular weight of 90 kDa and showed α D 20 −74.3288 (c 0.05, H2 O). SFPS65A is composed of fucose, galactose, xylose, glucose, glucuronic acid, and mannose in the ratio of 19.23:9.58:6.64:1:6.52:2.57. The structural features of SFPS65A were investigated using composition analysis, methylation analysis, infrared spectrum, nuclear magnetic resonance spectroscopy, and electrospray ionization quadruple time-of-flight tandem mass spectroscopy. Results showed that SFPS65A has a main chain composed of →3)- β -l -Fuc p -(1→3, 4)- β -l -Fuc p -(1→3, 4)- β -l -Fuc p -(1→ and connected with →3, 4)- α -d -GlcA p -(1→, →4)- β -d -Xyl p -(1→, →4)- α -d -Gal p -(1→, →3, 6)– α -d -Man p -(1→ alternately. The branches at O-3 of the fucosyl residue and O-3 of the hexosyl residues may include sulfate, →4)- β -l -Fuc p -(1→, β -d -Xyl p -(1→, and β -d -Xyl p -(1→. SFPS65A exhibited an activity on Alzheimer's disease in vivo in the pharmacological experiments by increasing the cognitive abilities of scopolamine-, ethanol-, and sodium nitrite-treated mice against memory deficits.