Low-concentration uranium enters the HepG2 cell nucleus rapidly and induces cell stress response. Issue 1 (25th December 2015)

• Record Type: Journal Article
• Authors: Guéguen, Yann; Suhard, David; Poisson, Clémentine; Manens, Line; Elie, Christelle; Landon, Géraldine; Bouvier-Capely, Céline; Rouas, Caroline; Benderitter, Marc; Tessier, Christine
• Is Part Of: Toxicology in vitro. Volume 30:Issue 1 Part B(2015)
• Page Start: 552
• Page End: 560
• Publication Date: 2015-12-25
• Subjects: APEX1 apurinic/apyrimidinic (AP) endodeoxyribonuclease -- BAX BCL2-associated X protein -- BID BH3 interacting domain death agonist -- CAT catalase -- DHE dihydroethidium -- DU depleted uranium -- HO-1 heme oxygenase -- ICP-MS inductively-coupled plasma mass spectrometry -- LDH lactate dehydrogenase -- NEIL endonuclease VIII-like DNA glycosylase -- NRF2 Nuclear Factor Erythroid 2-Related Factor -- OGG1 8-oxoguanine DNA glycosylase -- ROS reactive oxygen species -- SIMS secondary ion mass spectrometry -- SOD superoxide dismutase -- TEM transmission electronic microscopy; Uranium -- RedOx -- Localization -- Low-dose -- HepG2; Toxicity testing -- In vitro -- Periodicals; Toxicology -- Periodicals; 615.9
• DOI: 10.1016/j.tiv.2015.09.004
• Languages: English
• ISSNs: 0887-2333

Abstract

Abstract: This study aimed to compare the cell stress effects of low and high uranium concentrations and relate them to its localization, precipitate formation, and exposure time. The time-course analysis shows that uranium appears in cell nuclei as a soluble form within 5 min of exposure, and quickly induces expression of antioxidant and DNA repair genes. On the other hand, precipitate formations began at the very beginning of exposure at the 300-μM concentration, but took longer to appear at lower concentrations. Adaptive response might occur at low concentrations but are overwhelmed at high concentrations, especially when uranium precipitates are abundant. Highlights: SIMS is a new elemental imaging technique for uranium distribution in cells. Uranium enters cell nuclei very rapidly as a soluble form. Uranium quickly induces cell stress responses. Cell response leading to cell death is observed when uranium precipitates. Low-concentration uranium reinforces the antioxidant system.