Gallic acid induces mitotic catastrophe and inhibits centrosomal clustering in HeLa cells. Issue 1 (25th December 2015)

• Record Type: Journal Article
• Authors: Tan, Si; Guan, Xin; Grün, Christoph; Zhou, Zhiqin; Schepers, Ute; Nick, Peter
• Is Part Of: Toxicology in vitro. Volume 30:Issue 1 Part B(2015)
• Page Start: 506
• Page End: 513
• Publication Date: 2015-12-25
• Subjects: DMEM Dulbecco's modified Eagle's medium -- DMSO dimethyl sulfoxide -- FACS fluorescence-activated cell sorting -- FCS fetal calf serum -- GA gallic acid -- HeLa Human cervical cancer cell line -- HepG2 Human hepatoma cell line -- MAPs microtubule-associated proteins -- LD50 median lethal dose -- MT microtubule -- MTT 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide -- PBS−/− Phosphate-buffered saline without calcium and magnesium; Cell-cycle arrest -- Centrosome clustering -- Gallic acid (GA) -- HeLa -- Mitotic catastrophe; Toxicity testing -- In vitro -- Periodicals; Toxicology -- Periodicals; 615.9
• DOI: 10.1016/j.tiv.2015.09.011
• Languages: English
• ISSNs: 0887-2333

Abstract

Abstract: Cancer cells divide rapidly, providing medical targets for anticancer agents. The polyphenolic gallic acid (GA) is known to be toxic for certain cancer cells. However, the cellular mode of action has not been elucidated. Therefore, the current study addressed a potential effect of GA on the mitosis of cancer cells. GA inhibited viability of HeLa cells in a dose-dependent and time-dependent manner. We could show, using fluorescence-activated cell sorting (FACS), that this inhibition was accompanied by elevated frequency of cells arrested at the G2/M transition. This cell-cycle arrest was accompanied by mitotic catastrophe, and formation of cells with multiple nuclei. These aberrations were preceded by impaired centrosomal clustering. We arrive at a model of action, where GA inhibits the progression of the cell cycle at the G2/M phase by impairing centrosomal clustering which will stimulate mitotic catastrophe. Thus, GA has potential as compound against cervical cancer. Highlights: Gallic acid inhibited the cell viability and caused G2/M phase enrichment of HeLa. Gallic acid induced mitotic catastrophe of HeLa. Gallic acid may impair centrosome clustering of HeLa.