Identification of genes potentially related to herbivore resistance in OPR3 overexpression rice by microarray analysis. (October 2015)

• Record Type: Journal Article
• Authors: Guo, Hui-Min; Sun, Shi-Chen; Zhang, Feng-Ming; Miao, Xue-Xia
• Is Part Of: Physiological and molecular plant pathology. Volume 92(2015:Oct.)
• Page Start: 166
• Page End: 174
• Publication Date: 2015-10
• Subjects: Jasmonic acid (JA) -- cis-12-oxo-phytodienoic acid reductase 3 (OPR3) -- Biotic stress -- Defense related gene -- DNA repair gene -- Proliferation related gene; Plant diseases -- Periodicals; Diseased plants -- Physiology -- Periodicals; Phytopathogenic microorganisms -- Host plants -- Periodicals; 632
• DOI: 10.1016/j.pmpp.2015.05.008
• Languages: English
• ISSNs: 0885-5765

Abstract

Abstract: Cis-12-oxo-phytodienoic acid (OPDA) reductase 3 (OPR3) is a key enzyme in the jasmonic acid (JA) synthesis pathway. The OPR3 transcription level can be up-regulated by JA treatment, or by the rice striped stem borer's (SSB) feeding or mechanical wounding. To discover what facilitates defense functions in the JA pathway, we conducted a microarray analysis of OPR3 overexpressing rice plants ( OPR3ox ) and the wild-type Zhonghua 11. In total, 344 up- and 171 down-regulated genes were detected in OPR3ox plants. Of these, 67 genes were defense-related, and their expression patterns were consistent with JA, SSB or wounding treatments. Additionally, 61 DNA repair- and proliferation-related genes were detected among the OPR3ox up-regulated genes. However, most of these genes were down-regulated after JA, SSB, or wounding treatment. These genes may also have led to SSB resistance in the OPR3ox plants. Our results will help to further analyze the JA-mediated plant defense-response pathway and develop novel strategies to protect rice against biotic stresses. Highlights: We found 344 up- and 171 down-regulated genes in JA accumulating rice plants. Most defense-related genes are responsive to JA. DNA repair and proliferation genes are enhanced only in the OPR3ox rice line.