Isolation and characterization of two new diterpenoids from Stachys parviflora: Antidiarrheal potential in mice. (December 2015)

• Record Type: Journal Article
• Authors: Farooq, Umar; Naz, Sadia; Sarwar, Rizwana; Khan, Afsar; Khan, Ajmal; Rauf, Abdur; Khan, Haroon; Ahmad, Manzoor; Shah, Azhar-ul-Haq Ali; Hameed, Shaista
• Is Part Of: Phytochemistry letters. Volume 14(2015:Dec.)
• Page Start: 198
• Page End: 202
• Publication Date: 2015-12
• Subjects: Stachys parviflora -- Rosane diterpenoids -- Lamiaceae -- Antidiarrheal activity; Botanical chemistry -- Periodicals; Chimie végétale -- Périodiques; 572.205
• DOI: 10.1016/j.phytol.2015.10.001
• Languages: English
• ISSNs: 1874-3900

Abstract

Graphical abstract: Highlights: Two new diterpenoids were isolated from the ethyl acetate soluble fraction of Stachys parviflora. The structure elucidation of the compounds1 and2 were carried out by advanced spectroscopic analysis. These new diterpenoids1 and2 showed marked attenuation of castor oil induced diarrhea. Diterpenoids1 and2 could be useful antidiarrheal agent. Abstract: Two new diterpenoids trivially named Stachysrosane (1 ) and Stachysrosane (2 ) have been isolated from the ethyl acetate soluble fraction of Stachys parviflora . The structure elucidation of isolated diterpenoids were based on one-(1D) and two-dimensional (2D)-NMR techniques including correlation spectroscopy (COSY), heteronuclear multiple quantum coherence (HMQC), heteronuclear multiple bond correlation (HMBC), and nuclear Overhauser effect spectroscopy (NOESY). The isolated diterpenoids1 and2 caused marked attenuation of castor oil induced diarrhea in dose dependent manner with 100% and 70% protection at 45 mg/kg p.o. respectively. It can be concluded with confidence that both the isolated compounds could be useful antidiarrheal agents; however, detailed studies will be needed.