BIP induces mice CD19hi regulatory B cells producing IL-10 and highly expressing PD-L1, FasL. (January 2016)

• Record Type: Journal Article
• Authors: Tang, Youfa; Jiang, Qing; Ou, Yanghui; Zhang, Fan; Qing, Kai; Sun, Yuanli; Lu, Wenjie; Zhu, Huifen; Gong, Feili; Lei, Ping; Shen, Guanxin
• Is Part Of: Molecular immunology. Volume 69(2016:Jan.)
• Page Start: 44
• Page End: 51
• Publication Date: 2016-01
• Subjects: BIP -- Regulatory B cells -- IL-10 -- PD-L1 -- FasL; Immunochemistry -- Periodicals; Molecular biology -- Periodicals; Immunochemistry -- Periodicals; Allergy and Immunology -- Periodicals; Molecular Biology -- Periodicals; Immunochimie -- Périodiques; Biologie moléculaire -- Périodiques; Immunochemistry; Molecular biology; Periodicals; Electronic journals; 571.96
• DOI: 10.1016/j.molimm.2015.10.017
• Languages: English
• ISSNs: 0161-5890

Abstract

Highlights: BIP can induced IL-10 +, PD-L1 hi and FasL hi B cells. IL-10 +, PD-L1 hi and FasL hi B cells induced by BIP share the CD19 hi phenotype. CD40 signaling acts in synergy with BIP to induce regulatory B cells. Abstract: Many studies have shown that B cells possess a regulatory function in mouse models of autoimmune diseases. Regulatory B cells can modulate immune response through many types of molecular mechanisms, including the production of IL-10 and the expression of PD-1 Ligand and Fas Ligand, but the microenvironmental factors and mechanisms that induce regulatory B cells have not been fully identified. BIP (binding immunoglobulin protein), a member of the heat shock protein 70 family, is a type of evolutionarily highly conserved protein. In this article, we have found that IL-10 +, PD-L1 hi and FasL hi B cells are discrete cell populations, but enriched in CD19 hi cells. BIP can induce IL-10-producing splenic B cells, IL-10 secretion and B cells highly expressing PD-L1 and FasL. CD40 signaling acts in synergy with BIP to induce regulatory B cells. BIP increased surface CD19 molecule expression intensity and IL-10 +, PD-L1 hi and FasL hi B cells induced by BIP share the CD19 hi phenotype. Furthermore, B cells treated with BIP and anti-CD40 can lead to suppression of T cell proliferation and the effect is partially IL-10-dependent and mainly BIP-induced. Taken together, our findings identify a novel function of BIP in the induction of regulatory B cells and add a new reason for the therapy of autoimmune disorders or other inflammatory conditions.