Immunophenotype of mouse cerebral hemispheres-derived neural precursor cells. (12th January 2016)

• Record Type: Journal Article
• Authors: Poulatsidou, Kyriaki-Nefeli; Lagoudaki, Roza; Touloumi, Olga; Kesidou, Evangelia; Boziki, Marina; Ravanidis, Stylianos; Chlichlia, Katerina; Grigoriou, Maria; Grigoriadis, Nikolaos
• Is Part Of: Neuroscience letters. Volume 611(2016)
• Page Start: 33
• Page End: 39
• Publication Date: 2016-01-12
• Subjects: Mouse neural precursor cells -- CD molecules -- Flow cytometry -- Polysialylated neural adhesion molecule; Neurology -- Periodicals; Neurology -- Periodicals; Research -- Periodicals; Neurologie -- Périodiques; Neuroanatomie -- Périodiques; Neuropharmacologie -- Périodiques; Neurophysiologie -- Périodiques; Neurology; Periodicals; Electronic journals; 617.48
• DOI: 10.1016/j.neulet.2015.11.011
• Languages: English
• ISSNs: 0304-3940

Abstract

Graphical abstract: Highlights: Postnatally isolated NPCs (piNPCs) differentiated mainly into glial cells, in vitro. CD24 and CD44 though not CD49d were among the expressed cell adhesion molecules. Absence of CD184 and MHC expression indicate the piNPCs low antigenicity profile. Abstract: Postnatally isolated neural precursor cells (piNPCs) from mouse cerebral tissue have been studied in cell-based therapeutic approaches for Experimental Autoimmune Encephalomyelitis (EAE). Transplantation experiments in EAE rodents revealed that piNPCs manage to integrate into the host tissue and ameliorate clinical symptoms. When cultured in vitro, mouse cerebral piNPCs form neurospheres consisting of immature cells positive for polysialylated neural adhesion molecule (PSA-NCAM) that differentiate mainly towards glial cells, but also neurons. Herein, we have characterized piNPCs immunophenotype, with flow cytometry. NPCs were positive for CD24, CD44, and CD133 though negative for CD15, CD184 and CD49d. This immunophenotype, determined for the first time, among cells isolated from neonates might be useful for the identification of NPC population aiming at the development of transplantation protocols.