Administration of Lactobacillus helveticus NS8 improves behavioral, cognitive, and biochemical aberrations caused by chronic restraint stress. (3rd December 2015)

• Record Type: Journal Article
• Authors: Liang, S.; Wang, T.; Hu, X.; Luo, J.; Li, W.; Wu, X.; Duan, Y.; Jin, F.
• Is Part Of: Neuroscience. Volume 310(2015)
• Page Start: 561
• Page End: 577
• Publication Date: 2015-12-03
• Subjects: 5-HT serotonin -- ACTH adrenocorticotropic hormone -- BDNF brain-derived neurotrophic factor -- CIT citalopram -- CON control group -- CORT corticosterone group -- CRS chronic restraint stress group -- DA dopamine -- ELISA enzyme-linked immunosorbent assay -- EPM elevated plus-maze -- HPA hypothalamic–pituitary–adrenal -- IDO indoleamine-2, 3-dioxygenase -- IL-10 interleukin-10 -- INF-γ interferon-gama -- LB lactobacillus -- NE norepinephrine -- OFT open-field test -- OPT object placement test -- ORT object recognition test -- PFC prefrontal cortex -- qPCR quantitative real-time polymerase chain reaction -- SPF specific pathogen free -- SPT sucrose preference test -- SSRI selective serotonin reuptake inhibitor -- TDO tryptophan-2, 3-dioxygenase -- TNF-α tumor necrosis factor-alpha; Lactobacillus helveticus -- chronic restraint stress -- depression -- microbiota–gut–brain axis -- BDNF -- serotonin; Neurochemistry -- Periodicals; Neurophysiology -- Periodicals; Neurology -- Periodicals; Neurochimie -- Périodiques; Neurophysiologie -- Périodiques; Neurochemistry; Neurophysiology; Electronic journals; Periodicals; Electronic journals; 612.8
• DOI: 10.1016/j.neuroscience.2015.09.033
• Languages: English
• ISSNs: 0306-4522

Abstract

Graphical abstract: Highlights: The present study demonstrated LB was a very potential therapy in stress-related disorders. LB feeding resulted in antidepressant effect in CRS depression model as good as citalopram. LB feeding resulted in better anxiolytic and cognition promotion effect than citalopram. LB feeding improved behaviors and memory possibly through nerve, immune, and endocrine routes. Abstract: Increasing numbers of studies have suggested that the gut microbiota is involved in the pathophysiology of stress-related disorders. Chronic stress can cause behavioral, cognitive, biochemical, and gut microbiota aberrations. Gut bacteria can communicate with the host through the microbiota–gut–brain axis (which mainly includes the immune, neuroendocrine, and neural pathways) to influence brain and behavior. It is hypothesized that administration of probiotics can improve chronic-stress-induced depression. In order to examine this hypothesis, the chronic restraint stress depression model was established in this study. Adult specific pathogen free (SPF) Sprague–Dawley rats were subjected to 21 days of restraint stress followed by behavioral testing (including the sucrose preference test (SPT), elevated-plus maze test, open-field test (OFT), object recognition test (ORT), and object placement test (OPT)) and biochemical analysis. Supplemental Lactobacillus helveticus NS8 was provided every day during stress until the end of experiment, and selective serotonin reuptake inhibitor (SSRI) citalopram (CIT) served as a positive control. Results showed that L. helveticus NS8 improved chronic restraint stress-induced behavioral (anxiety and depression) and cognitive dysfunction, showing an effect similar to and better than that of CIT. L. helveticus NS8 also resulted in lower plasma corticosterone (CORT) and adrenocorticotropic hormone (ACTH) levels, higher plasma interleukin-10 (IL-10) levels, restored hippocampal serotonin (5-HT) and norepinephrine (NE) levels, and more hippocampal brain-derived neurotrophic factor (BDNF) mRNA expression than in chronic stress rats. Taken together, these results indicate an anti-depressant effect of L. helveticus NS8 in rats subjected to chronic restraint stress depression and that this effect could be due to the microbiota–gut–brain axis. They also suggest the therapeutic potential of L. helveticus NS8 in stress-related and possibly other kinds of depression.