The effects of hypoxia-inducible factor (HIF)-1α protein on bone regeneration during distraction osteogenesis: an animal study. Issue 2 (February 2016)

• Record Type: Journal Article
• Authors: Jiang, X.; Zhang, Y.; Fan, X.; Deng, X.; Zhu, Y.; Li, F.
• Is Part Of: International journal of oral & maxillofacial surgery. Volume 45:Issue 2(2016:Feb.)
• Page Start: 267
• Page End: 272
• Publication Date: 2016-02
• Subjects: hypoxia-inducible factor -- distraction osteogenesis -- rabbit -- mandible; Mouth -- Surgery -- Periodicals; Maxilla -- Surgery -- Periodicals; Dentistry -- Periodicals; Dentistry, Operative; Oral Surgical Procedures; Surgery, Oral; Dentistry; Maxilla -- Surgery; Mouth -- Surgery; Electronic journals; Periodicals; 617.52059
• DOI: 10.1016/j.ijom.2015.09.021
• Languages: English
• ISSNs: 0901-5027

Abstract

Abstract: The aim of this study was to observe the effect of hypoxia-inducible factor (HIF)-1α on bone regeneration during distraction osteogenesis (DO). Fifty-one New Zealand white rabbits underwent mandibular lengthening with a distraction rate of 2 mm/day, and were divided randomly into three groups (17 in each). Group C rabbits received 20 μg rHIF-1α, group B received 10 μg rHIF-1α, and group A received 100 μl saline injection in the distraction gap every day for 10 days. Radionuclide bone imaging (RBI), computed tomography, dual energy X-ray absorptiometry, radiography, histology, and three-point bend testing were performed. RBI showed that the uptake ratio in group B (1.41 ± 0.25, P = 0.013) and group C (1.64 ± 0.37, P < 0.001) was higher than that in group A (1.01 ± 0.26). The bone mineralization density and bone mineralization content in group C were highest among the three groups. Radiology and histology findings indicated more callus regeneration in groups C and B. Mechanical testing demonstrated that the ultimate force in group C (289.71 ± 43.31 N, n = 6) was 1.49-fold ( P < 0.001) that of group A and 1.20-fold ( P = 0.012) that of group B. HIF-1α may represent a new agent to promote DO by accelerating osteogenesis and mineralization.