Metabolic profiles of placenta in preeclampsia using HR-MAS MRS metabolomics. Issue 12 (December 2015)

• Record Type: Journal Article
• Authors: Austdal, Marie; Thomsen, Liv Cecilie Vestrheim; Tangerås, Line Haugstad; Skei, Bente; Mathew, Seema; Bjørge, Line; Austgulen, Rigmor; Bathen, Tone Frost; Iversen, Ann-Charlotte
• Is Part Of: Placenta. Volume 36:Issue 12(2015:Dec.)
• Page Start: 1455
• Page End: 1462
• Publication Date: 2015-12
• Subjects: Preeclampsia -- Metabolomics -- Placenta -- Metabolism -- Profiling -- NMR; Placenta -- Periodicals; Reproduction -- Periodicals; Placenta -- Periodicals; Placenta -- Périodiques; Reproduction -- Périodiques; 612.63
• DOI: 10.1016/j.placenta.2015.10.019
• Languages: English
• ISSNs: 0143-4004

Abstract

Abstract: Introduction: Preeclampsia is a heterogeneous gestational disease characterized by maternal hypertension and proteinuria, affecting 2–7% of pregnancies. The disorder is initiated by insufficient placental development, but studies characterizing the placental disease components are lacking. Methods: Our aim was to phenotype the preeclamptic placenta using high-resolution magic angle spinning nuclear magnetic resonance spectroscopy (HR-MAS MRS). Placental samples collected after delivery from women with preeclampsia (n = 19) and normotensive pregnancies (n = 15) were analyzed for metabolic biomarkers including amino acids, osmolytes, and components of the energy and phospholipid metabolism. The metabolic biomarkers were correlated to clinical characteristics and inflammatory biomarkers in the maternal sera. Results: Principal component analysis showed inherent differences in placental metabolic profiles between preeclamptic and normotensive pregnancies. Significant differences in metabolic profiles were found between placentas from severe and non-severe preeclampsia, but not between preeclamptic pregnancies with fetal growth restricted versus normal weight neonates. The placental metabolites correlated with the placental stress marker sFlt-1 and triglycerides in maternal serum, suggesting variation in placental stress signaling between different placental phenotypes. Discussion: HR-MAS MRS is a sensitive method for defining the placental disease component of preeclampsia, identifying several altered metabolic pathways. Placental HR-MAS MRS analysis may improve insight into processes affected in the preeclamptic placenta, and represents a novel long-required tool for a sensitive placental phenotyping of this heterogeneous disease. Graphical abstract: Highlights: The largest metabolic profiling of preeclamptic placentas to date. Metabolic pathways are differently expressed in preeclamptic and normotensive placentas. Metabolite changes in preeclampsia involve taurine and phospholipids. Metabolic profiles correlate to maternal serum measurements. Novel, sensitive, and nondestructive method for placental tissue profiling.