A portable optical waveguide resonance light-scattering scanner for microarray detection. Issue 1 (16th November 2015)

• Record Type: Journal Article
• Authors: Xing, Xuefeng; Liu, Wanyao; Li, Tao; Xing, Shu; Fu, Xueqi; Wu, Dongyang; Liu, Dianjun; Wang, Zhenxin
• Is Part Of: Analyst. Volume 141:Issue 1(2016)
• Page Start: 199
• Page End: 205
• Publication Date: 2015-11-16
• Subjects: Chemistry, Analytic -- Periodicals; 543
• DOI: 10.1039/c5an01839k
• Languages: English
• ISSNs: 0003-2654

Abstract

Abstract : A portable and low-cost planar waveguide based RLS microarray scanner was successfully fabricated to analyze 15 T2DM patient genotypes simultaneously. Abstract : In the present work, a portable and low-cost planar waveguide based resonance light scattering (RLS) scanner (termed as: PW-RLS scanner) has been developed for microarray detection. The PW-RLS scanner employs a 2 × 4 white light emitting diode array (WLEDA) as the excitation light source, a folded optical path with a complementary metal oxide semiconductor (CMOS) as the signal/image acquisition device and stepper motors with gear drives as the mechanical drive system. The biological binding/recognizing events on the microarray can be detected with an evanescent waveguide-directed illumination and light-scattering label ( e.g., nanoparticles) while the microarray slide acts as an evanescent waveguide substrate. The performance of the as-developed PW-RLS scanner has been evaluated by analyzing type 2 diabetes mellitus (T2DM) risk genes. Highly selective and sensitive (less than 1% allele frequency at the attomole-level) T2DM risk gene detection is achieved using single-stranded DNA functionalized gold nanoparticles (ssDNA-GNPs) as detection probes. Additionally, the successful simultaneous analysis of 15 T2DM patient genotypes suggests that the device has great potential for the realization of a personalized diagnostic test for a given disease or patient follow-up.