A 28-day oral gavage toxicity study of 3-monochloropropane-1, 2-diol (3-MCPD) in CB6F1-non-Tg rasH2 mice. (December 2015)

• Record Type: Journal Article
• Authors: Lee, Byoung-Seok; Park, Sang-Jin; Kim, Yong-Bum; Han, Ji-Seok; Jeong, Eun-Ju; Moon, Kyoung-Sik; Son, Hwa-Young
• Is Part Of: Food and chemical toxicology. Volume 86(2015)
• Page Start: 95
• Page End: 103
• Publication Date: 2015-12
• Subjects: 3-MCPD -- Toxicological pathology -- Risk assessment -- CB6F1-non-Tg rasH2 Mice; Toxicology -- Periodicals; Food poisoning -- Periodicals; Food Poisoning -- Periodicals; Toxicology -- Periodicals; Toxicologie -- Périodiques; Intoxications alimentaires -- Périodiques; Food poisoning; Toxicology; Periodicals; Electronic journals; 615.9
• DOI: 10.1016/j.fct.2015.09.019
• Languages: English
• ISSNs: 0278-6915

Abstract

Abstract: 3-Monochloro-1, 2-propanediol (3-MCPD) is a well-known contaminant of foods containing hydrolyzed vegetable protein. However, limited toxicity data are available for the risk assessment of 3-MCPD and its carcinogenic potential is controversial. To evaluate the potential toxicity and determine the dose levels for a 26-week carcinogenicity test using Tg rasH2 mice, 3-MCPD was administered once daily by oral gavage at doses of 0, 25, 50, and 100 mg/kg body weight (b.w.)/day for 28 days to male and female CB6F1-non-Tg rasH2 mice (N = 5 males and females per dose). The standard toxicological evaluations were conducted during the in-life and post-mortem phase. In the 100 mg/kg b.w./day group, 3 males and 1 female died during the study and showed clinical signs such as thin appearance and subdued behavior accompanied by significant decreases in mean b.w. Microscopy revealed tubular basophilia in the kidneys, exfoliated degenerative germ cells in the lumen of the seminiferous tubule of the testes, vacuolation in the brain, axonal degeneration of the sciatic nerve, and cardiomyopathy in the 100, ≥25, ≥50, 100, and 100 mg/kg b.w./day groups, respectively. In conclusion, 3-MCPD's target organs were the kidneys, testes, brain, sciatic nerve, and heart. The "no-observed-adverse-effect level" (NOAEL) of 3-MCPD was ≤25 and 25 mg/kg b.w./day in males and females, respectively. Highlights: A 28-day oral gavage toxicity of 3-monochloropropane-1, 2-diol (3-MCPD) was investigated in mice. 28-day oral toxicity of 3-MCPD have a toxicity on the renal and reproductive system as well with its neurotoxic, immunotoxic, and cardiotoxic effects in mice. The "no-observed-adverse-effect level" (NOAEL) of 3-MCPD was ≤25 and 25 mg/kg b.w./day in males and females, respectively.