Aerosol from a candidate modified risk tobacco product has reduced effects on chemotaxis and transendothelial migration compared to combustion of conventional cigarettes. (December 2015)

• Record Type: Journal Article
• Authors: van der Toorn, Marco; Frentzel, Stefan; De Leon, Hector; Goedertier, Didier; Peitsch, Manuel C.; Hoeng, Julia
• Is Part Of: Food and chemical toxicology. Volume 86(2015)
• Page Start: 81
• Page End: 87
• Publication Date: 2015-12
• Subjects: Transendothelial migration -- THP-1 cells -- Endothelial cells -- Atherosclerosis -- Modified risk tobacco product -- Tobacco-heating system; 7-AAD 7-aminoactinomycin D -- BSA bovine serum albumin -- CIM cell invasion/migration -- CXCL12 C-X-C motif ligand 12 -- HCAECs human coronary arterial endothelial cells -- IL-8 Interleukin-8 -- MRTP modified risk tobacco product -- RTCA real-time cell analyzer -- TEM transendothelial migration -- THS tobacco-heating system -- TNF-α tumor necrosis factor-α; Toxicology -- Periodicals; Food poisoning -- Periodicals; Food Poisoning -- Periodicals; Toxicology -- Periodicals; Toxicologie -- Périodiques; Intoxications alimentaires -- Périodiques; Food poisoning; Toxicology; Periodicals; Electronic journals; 615.9
• DOI: 10.1016/j.fct.2015.09.016
• Languages: English
• ISSNs: 0278-6915

Abstract

Abstract: Reduction of harmful constituents by heating rather than combusting tobacco is a promising new approach to reduce harmful effects associated with cigarette smoking. We investigated the effect from a new candidate modified risk tobacco product, the tobacco heating system (THS) 2.2, on the migratory behavior of monocytes in comparison with combustible 3R4F reference cigarettes. The monocytic cell line (THP-1) and human coronary arterial endothelial cells (HCAECs) were used to analyze chemotaxis and transendothelial migration (TEM). To assess the influence of aerosol extract from THS2.2 and smoke extract from 3R4F on toxicity and inflammation, flow cytometry and ELISA assays were performed. The results show that treatment of THP-1 cells with extract from 3R4F or THS2.2 induced concentration-dependent increases in cytotoxicity and inflammation. The inhibitory effects of THS2.2 extract for chemotaxis and TEM were ∼18 times less effective compared to 3R4F extract. Furthermore, extract from 3R4F or THS2.2 induced concentration-dependent decreases in the integrity of HCAEC monolayer. For all examined endpoints, the extract from 3R4F showed more than one order of magnitude stronger effects than that from THS2.2 extract. These data indicate the potential of a heat not burn tobacco product to reduce the risk for cardiovascular disease compared to combustible cigarettes. Highlights: Transendothelial migration of monocytes is accompanied by significant changes in adhesion and expression of MAC-1. Extracts from the tobacco heating system (THS) 2.2 induce less inflammation and migration and are less cytotoxic than those from burning conventional cigarettes. Reduction in the exposure to harmful constituents using heated rather than burning tobacco could be a promising new approach to reduce the harmful effects associated with cigarette smoking.