Ultrasensitive electrochemical detection of UO22+ based on DNAzyme and isothermal enzyme-free amplification. Issue 5 (6th January 2016)

• Record Type: Journal Article
• Authors: Yun, Wen; Jiang, Jiaolai; Cai, Dingzhou; Wang, Xiaofang; Sang, Ge; Liao, Junsheng; Lu, Tiecheng; Yan, Kangping
• Is Part Of: RSC advances. Volume 6:Issue 5(2016)
• Page Start: 3960
• Page End: 3966
• Publication Date: 2016-01-06
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/c5ra22773a
• Languages: English
• ISSNs: 2046-2069

Abstract

Abstract : A novel enzyme-free amplification biosensor for uranyl detection was developed based on UO2 2+ -specific DNAzyme and a hybridization chain reaction. Abstract : A novel strategy for ultrasensitive detection of UO2 2+ was proposed by using a UO2 2+ -specific DNAzyme and two free labeled DNA hairpins. The principle and detection processes of this electrochemical biosensor for amplification detection of UO2 2+ were described. The detection conditions of the biosensor, such as the concentration of hairpins, pH, and the incubation time of HCR, were optimized. Under optimal conditions, this biosensor demonstrated a high sensitivity of UO2 2+ detection with the dynamic concentration range spanning from 0.05 to 4 nM and a detection limit of 20 pM at the 3Sblank level, providing a promising strategy for on site and real-time monitoring of uranium.