Keratinocyte growth factor-2 inhibits bacterial infection with Pseudomonas aeruginosa pneumonia in a mouse model. Issue 1 (January 2016)

• Record Type: Journal Article
• Authors: Feng, Nana; Wang, Qin; Zhou, Jian; Li, Jing; Wen, Xiaoxing; Chen, Shujing; Zhu, Zhenhua; Bai, Chunxue; Song, Yuanlin; Li, Huayin
• Is Part Of: Journal of infection and chemotherapy. Volume 22:Issue 1(2016:Jan.)
• Page Start: 44
• Page End: 52
• Publication Date: 2016-01
• Subjects: Keratinocyte growth factor-2 -- Pseudomonas aeruginosa -- Pneumonia -- Lung injury; Chemotherapy -- Periodicals; Infection -- Periodicals; Communicable diseases -- Chemotherapy -- Periodicals; 615.5805
• DOI: 10.1016/j.jiac.2015.10.005
• Languages: English
• ISSNs: 1341-321X

Abstract

Abstract: To determine protective effects of concurrent administration of Keratinocyte growth factor-2 (KGF-2) with Pseudomonas aeruginosa (P . aeruginosa) inoculation on the induced pneumonia. KGF-2 (5 mg/kg) was concurrently administered into the left lobe of 55 mice with P . aeruginosa PAO1 (5 × 10 6 CFU, half-lethal dose); 55 mice in the control group were concurrently administered PBS with the PAO1. We detected and analyzed: body temperature; amount of P . aeruginosa in homogenates; count of total number of nucleated cells and of mononuclear macrophages; protein concentration in bronchoalveolar lavage fluid (BALF); lung wet-to-dry weight ratio; cytokines in BALF and blood; and lung morphology. To study survival rate, concurrent administration of KGF-2 (experimental group) versus PBS (control) with a lethal dose of PAO1 (1 × 10 7 CFU was performed, and survivorship was documented for 7 days post-inoculation. The bacterial CFU in lung homogenates was significantly decreased in the KGF-2 group compared to the control group. There were significantly more mononuclear macrophages in the BALF from the KGF-2 group than from the control group (p < 0.05). KGF-2 increased the surfactant protein and GM-CSF mRNA in lung at 6 h and 72 h after inoculation. Significant reduction of lung injury scores, protein concentrations, lung wet-to-dry weight ratio, and IL-6 and TNF-α levels was noted in the KGF-2 treated rats at 72 h after inoculation (p < 0.05). The 7-day survival rate of the KGF-2 group was significantly higher than that of the control group (p < 0.05). Concurrent administration of KGF-2 facilitates the clearance of P . aeruginosa from the lungs, attenuates P . aeruginosa -induced lung injury, and extends the 7-day survival rate in mice model with P . aeruginosa pneumonia.